Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30540 | 3' | -54.8 | NC_006548.1 | + | 14646 | 0.66 | 0.595567 |
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Target: 5'- cAGCgucGCGAuGUUcuuugCCGCugACGCCAGCAg -3' miRNA: 3'- -UUGa--CGCUcUAAa----GGCG--UGCGGUCGU- -5' |
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| 30540 | 3' | -54.8 | NC_006548.1 | + | 36241 | 0.67 | 0.538673 |
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Target: 5'- gGACUGCG-GAcgUCCcgGC-UGCCGGCAg -3' miRNA: 3'- -UUGACGCuCUaaAGG--CGuGCGGUCGU- -5' |
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| 30540 | 3' | -54.8 | NC_006548.1 | + | 17297 | 0.67 | 0.516414 |
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Target: 5'- ---aGCGGGAUgaaaaggugUUCCGCAUGUCAGg- -3' miRNA: 3'- uugaCGCUCUA---------AAGGCGUGCGGUCgu -5' |
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| 30540 | 3' | -54.8 | NC_006548.1 | + | 35876 | 0.67 | 0.516414 |
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Target: 5'- uGCcgGCGcGA--UCCGCGCGgCCGGCGg -3' miRNA: 3'- uUGa-CGCuCUaaAGGCGUGC-GGUCGU- -5' |
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| 30540 | 3' | -54.8 | NC_006548.1 | + | 1119 | 0.68 | 0.462536 |
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Target: 5'- ---aGCGAGccuggccgUCUGCGCGgCCAGCAa -3' miRNA: 3'- uugaCGCUCuaa-----AGGCGUGC-GGUCGU- -5' |
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| 30540 | 3' | -54.8 | NC_006548.1 | + | 28395 | 0.68 | 0.441821 |
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Target: 5'- cACcgGCGGGAccgUUCaCGCGCGCC-GCAa -3' miRNA: 3'- uUGa-CGCUCUa--AAG-GCGUGCGGuCGU- -5' |
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| 30540 | 3' | -54.8 | NC_006548.1 | + | 12322 | 0.7 | 0.355606 |
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Target: 5'- cGACggGCGAGcgggCaucaGCACGCCAGCGg -3' miRNA: 3'- -UUGa-CGCUCuaaaGg---CGUGCGGUCGU- -5' |
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| 30540 | 3' | -54.8 | NC_006548.1 | + | 37791 | 0.7 | 0.338125 |
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Target: 5'- gAACaGCGAGuagacgCCGguCGCCGGCAc -3' miRNA: 3'- -UUGaCGCUCuaaa--GGCguGCGGUCGU- -5' |
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| 30540 | 3' | -54.8 | NC_006548.1 | + | 23737 | 1.06 | 0.000819 |
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Target: 5'- cAACUGCGAGAUUUCCGCACGCCAGCAg -3' miRNA: 3'- -UUGACGCUCUAAAGGCGUGCGGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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