Results 41 - 50 of 50 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30542 | 3' | -54.2 | NC_006548.1 | + | 7403 | 0.68 | 0.50934 |
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Target: 5'- aUGCCCAGUucGCGGACgGAGUGGgaGCCu -3' miRNA: 3'- gACGGGUUAc-CGUUUGaCUCGUC--CGG- -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 7593 | 0.68 | 0.498429 |
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Target: 5'- aCUGCgUGAaGGCuucgccaaguuGGCcGAGCAGGCCg -3' miRNA: 3'- -GACGgGUUaCCGu----------UUGaCUCGUCCGG- -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 33296 | 0.68 | 0.487624 |
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Target: 5'- -cGCCUggUcGGCAAAUUGcGGCAGcCCg -3' miRNA: 3'- gaCGGGuuA-CCGUUUGAC-UCGUCcGG- -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 12334 | 0.69 | 0.455901 |
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Target: 5'- -gGCCCuGUGcGCcGACgggcGAGCGGGCa -3' miRNA: 3'- gaCGGGuUAC-CGuUUGa---CUCGUCCGg -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 27093 | 0.7 | 0.396001 |
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Target: 5'- -cGCCaCcAUGGCc--CUGAuGCGGGCCg -3' miRNA: 3'- gaCGG-GuUACCGuuuGACU-CGUCCGG- -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 15160 | 0.71 | 0.350126 |
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Target: 5'- -cGgCCAuUGGCGAACUccagGAcGUAGGCCg -3' miRNA: 3'- gaCgGGUuACCGUUUGA----CU-CGUCCGG- -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 23117 | 0.71 | 0.332865 |
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Target: 5'- --aCCCAAcGGCAgcAACUGAGCAG-CCu -3' miRNA: 3'- gacGGGUUaCCGU--UUGACUCGUCcGG- -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 21861 | 0.73 | 0.277435 |
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Target: 5'- uCUGCUCGGUGGCAuGGCcGAGgGGGUUg -3' miRNA: 3'- -GACGGGUUACCGU-UUGaCUCgUCCGG- -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 15968 | 0.74 | 0.22962 |
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Target: 5'- -gGUCCuccAUGGCGccgcgGAUUGGGUAGGCCa -3' miRNA: 3'- gaCGGGu--UACCGU-----UUGACUCGUCCGG- -5' |
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| 30542 | 3' | -54.2 | NC_006548.1 | + | 23406 | 0.76 | 0.159276 |
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Target: 5'- uUGgCCAGUGGCAGACUGuAGCcccGGCg -3' miRNA: 3'- gACgGGUUACCGUUUGAC-UCGu--CCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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