Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30542 | 5' | -56.3 | NC_006548.1 | + | 14257 | 0.66 | 0.538137 |
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Target: 5'- aGggGCGccugGCCgauGGCCggaUGACCCUGgAc -3' miRNA: 3'- -CuuUGCa---CGG---CCGGa--ACUGGGACgUc -5' |
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| 30542 | 5' | -56.3 | NC_006548.1 | + | 24378 | 0.66 | 0.527189 |
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Target: 5'- cAGGCGcucGCCGGCCcaGACCCgGCc- -3' miRNA: 3'- cUUUGCa--CGGCCGGaaCUGGGaCGuc -5' |
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| 30542 | 5' | -56.3 | NC_006548.1 | + | 18587 | 0.67 | 0.48431 |
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Target: 5'- -cAGCGUGCCGaCaugGACCCgGCGGc -3' miRNA: 3'- cuUUGCACGGCcGgaaCUGGGaCGUC- -5' |
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| 30542 | 5' | -56.3 | NC_006548.1 | + | 22092 | 0.68 | 0.413646 |
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Target: 5'- -cAugGUGCCgauGGCCU---UCCUGCAGg -3' miRNA: 3'- cuUugCACGG---CCGGAacuGGGACGUC- -5' |
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| 30542 | 5' | -56.3 | NC_006548.1 | + | 19956 | 0.68 | 0.404076 |
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Target: 5'- -cGACGUGCCGGCCggcggggagGAUUg-GCAGa -3' miRNA: 3'- cuUUGCACGGCCGGaa-------CUGGgaCGUC- -5' |
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| 30542 | 5' | -56.3 | NC_006548.1 | + | 13690 | 0.69 | 0.358389 |
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Target: 5'- cAGGCGUGCCuGGUCUUGG-CUUGCGa -3' miRNA: 3'- cUUUGCACGG-CCGGAACUgGGACGUc -5' |
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| 30542 | 5' | -56.3 | NC_006548.1 | + | 20839 | 0.73 | 0.207402 |
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Target: 5'- cAGGCG-GCCGGCCUUGACCagGCc- -3' miRNA: 3'- cUUUGCaCGGCCGGAACUGGgaCGuc -5' |
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| 30542 | 5' | -56.3 | NC_006548.1 | + | 5845 | 0.75 | 0.148392 |
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Target: 5'- cGAACGgaaCGGCCUUGGCCUUGCGc -3' miRNA: 3'- cUUUGCacgGCCGGAACUGGGACGUc -5' |
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| 30542 | 5' | -56.3 | NC_006548.1 | + | 24374 | 1.09 | 0.000462 |
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Target: 5'- gGAAACGUGCCGGCCUUGACCCUGCAGa -3' miRNA: 3'- -CUUUGCACGGCCGGAACUGGGACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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