miRNA: 3'- -GAUGCGuaCGG---AGUAGCg-------ACCUCCua -5'
miRNA: 3'- gAUGCgUACGG-----AGU---AGCGACCUCCua -5'
miRNA: 3'- -GAUG-CGUACGGAgUaGCGACCUccua -5'
miRNA: 3'- -GAUGCGUa-------CGGa-GUAGCGACC---UCCUa -5'
miRNA: 3'- -GAUGCGUACGGAGUAGCGACCUCCUA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30545 | 5' | -55.3 | NC_006548.1 | + | 1130 | 0.66 | 0.567788 |
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Target: 5'- gCUGCGCcgGCUgggUCGUCGacauggcaUGGAGGu- -3' miRNA: 3'- -GAUGCGuaCGG---AGUAGCg-------ACCUCCua -5' |
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| 30545 | 5' | -55.3 | NC_006548.1 | + | 12507 | 0.66 | 0.534128 |
|
Target: 5'- gUACGaAUGCCcagcgUCAcacUCGCUGGAGGc- -3' miRNA: 3'- gAUGCgUACGG-----AGU---AGCGACCUCCua -5' |
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| 30545 | 5' | -55.3 | NC_006548.1 | + | 19472 | 0.66 | 0.534128 |
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Target: 5'- gCUGCuGCGUGCCUgG-CGCUGGAu--- -3' miRNA: 3'- -GAUG-CGUACGGAgUaGCGACCUccua -5' |
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| 30545 | 5' | -55.3 | NC_006548.1 | + | 18207 | 0.67 | 0.469201 |
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Target: 5'- cCUACGCGgaggacgaGCUggCAUCGCUGGgcaAGGAc -3' miRNA: 3'- -GAUGCGUa-------CGGa-GUAGCGACC---UCCUa -5' |
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| 30545 | 5' | -55.3 | NC_006548.1 | + | 25497 | 1.06 | 0.00082 |
|
Target: 5'- uCUACGCAUGCCUCAUCGCUGGAGGAUa -3' miRNA: 3'- -GAUGCGUACGGAGUAGCGACCUCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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