Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30551 | 3' | -52.7 | NC_006548.1 | + | 29134 | 0.66 | 0.760283 |
Target: 5'- aCUCGACuCCuGCCGAGaCUGcUCaCCGa -3' miRNA: 3'- -GAGUUGuGGuCGGUUCgGAUaAG-GGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 2363 | 0.66 | 0.760283 |
Target: 5'- ---cGCACCcGCCAGGCCggcgguUUUCCa -3' miRNA: 3'- gaguUGUGGuCGGUUCGGau----AAGGGc -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 33962 | 0.66 | 0.749431 |
Target: 5'- gUCGGCACUGGCCuGGCCggucaugCgCCGu -3' miRNA: 3'- gAGUUGUGGUCGGuUCGGauaa---G-GGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 16850 | 0.66 | 0.738446 |
Target: 5'- --gAACACCGGCCAguacagccaaugGGCCagg-CCCa -3' miRNA: 3'- gagUUGUGGUCGGU------------UCGGauaaGGGc -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 20226 | 0.66 | 0.738446 |
Target: 5'- cCUCggUGCCGuGCCGccGGCCaucaaaggcAUUCCCGa -3' miRNA: 3'- -GAGuuGUGGU-CGGU--UCGGa--------UAAGGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 8702 | 0.66 | 0.727343 |
Target: 5'- ---cGCugCGGgCGGGCCUGgagUCCCu -3' miRNA: 3'- gaguUGugGUCgGUUCGGAUa--AGGGc -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 29075 | 0.66 | 0.727343 |
Target: 5'- -gCAGC-UCAGCCuuGGCCUucucgAUUCCCa -3' miRNA: 3'- gaGUUGuGGUCGGu-UCGGA-----UAAGGGc -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 38059 | 0.66 | 0.727343 |
Target: 5'- aUCAaaACGCUuaGGCCAGGCgCgcaaUCCCGa -3' miRNA: 3'- gAGU--UGUGG--UCGGUUCG-Gaua-AGGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 20785 | 0.66 | 0.716134 |
Target: 5'- gUCAAgGCCGGCC--GCCUGcUgCCGc -3' miRNA: 3'- gAGUUgUGGUCGGuuCGGAUaAgGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 22737 | 0.66 | 0.704833 |
Target: 5'- gCUCGAuCGCCAGCguGGCggcgUCCUGg -3' miRNA: 3'- -GAGUU-GUGGUCGguUCGgauaAGGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 35495 | 0.67 | 0.693454 |
Target: 5'- uUCAccugguCGCCGGCCAGGCUgaucacugcuUUCUCGg -3' miRNA: 3'- gAGUu-----GUGGUCGGUUCGGau--------AAGGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 1883 | 0.67 | 0.678565 |
Target: 5'- uUCAcgucACACCcccugaguaagccgAGCCAcGGCCUGgugCCCGa -3' miRNA: 3'- gAGU----UGUGG--------------UCGGU-UCGGAUaa-GGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 29832 | 0.67 | 0.670512 |
Target: 5'- -gCAAC-CCAGCCGGGCaugacCCCGc -3' miRNA: 3'- gaGUUGuGGUCGGUUCGgauaaGGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 14413 | 0.67 | 0.647409 |
Target: 5'- -cCAGCACCgaggcGGCCAAGCUgauccgugCCCa -3' miRNA: 3'- gaGUUGUGG-----UCGGUUCGGauaa----GGGc -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 32399 | 0.67 | 0.647409 |
Target: 5'- aUCAGCcugGCCGGCC-AGCCg--UCgCCGc -3' miRNA: 3'- gAGUUG---UGGUCGGuUCGGauaAG-GGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 32207 | 0.69 | 0.564371 |
Target: 5'- gUCGagGCGCUGGCCGAGCCcagggugaugUCCUGg -3' miRNA: 3'- gAGU--UGUGGUCGGUUCGGaua-------AGGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 8397 | 0.69 | 0.543959 |
Target: 5'- uCUCuACGCuCGGCCAGGCCgg--CCuCGa -3' miRNA: 3'- -GAGuUGUG-GUCGGUUCGGauaaGG-GC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 29693 | 0.69 | 0.532722 |
Target: 5'- -gCGGCACCAGCaccugCGAGCCcAUUCgCGg -3' miRNA: 3'- gaGUUGUGGUCG-----GUUCGGaUAAGgGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 32976 | 0.69 | 0.532722 |
Target: 5'- -gCGGCGCUGGCC-GGCCggcUCCCGc -3' miRNA: 3'- gaGUUGUGGUCGGuUCGGauaAGGGC- -5' |
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30551 | 3' | -52.7 | NC_006548.1 | + | 25765 | 0.7 | 0.499548 |
Target: 5'- -cCAGC-CCAGCCAAGgCCc--UCCCGc -3' miRNA: 3'- gaGUUGuGGUCGGUUC-GGauaAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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