Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30552 | 5' | -56.1 | NC_006548.1 | + | 18445 | 0.67 | 0.445575 |
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Target: 5'- gUGGcAGCGGcGUGGUCuucuGCA-GGGCGc -3' miRNA: 3'- gACC-UCGUC-UACCAGu---CGUuCCCGUu -5' |
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| 30552 | 5' | -56.1 | NC_006548.1 | + | 9292 | 0.67 | 0.424325 |
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Target: 5'- uCUGGAgacgcugGCGGAUGcUCAGCc-GGGCGAu -3' miRNA: 3'- -GACCU-------CGUCUACcAGUCGuuCCCGUU- -5' |
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| 30552 | 5' | -56.1 | NC_006548.1 | + | 1460 | 0.68 | 0.396001 |
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Target: 5'- aCUGGuucAGCAGuuccUGGcCGGCGGcGGGCAGa -3' miRNA: 3'- -GACC---UCGUCu---ACCaGUCGUU-CCCGUU- -5' |
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| 30552 | 5' | -56.1 | NC_006548.1 | + | 25996 | 0.7 | 0.292482 |
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Target: 5'- -aGGAGgcucucggcccuCAGAUGGUuaaucgccccgCAGCGGGGGCAu -3' miRNA: 3'- gaCCUC------------GUCUACCA-----------GUCGUUCCCGUu -5' |
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| 30552 | 5' | -56.1 | NC_006548.1 | + | 14696 | 0.71 | 0.242526 |
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Target: 5'- gCUGGAuGC-GAUGccCAGCAAGGGCGGa -3' miRNA: 3'- -GACCU-CGuCUACcaGUCGUUCCCGUU- -5' |
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| 30552 | 5' | -56.1 | NC_006548.1 | + | 27859 | 1.06 | 0.000556 |
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Target: 5'- cCUGGAGCAGAUGGUCAGCAAGGGCAAg -3' miRNA: 3'- -GACCUCGUCUACCAGUCGUUCCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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