Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30556 | 3' | -50.4 | NC_006548.1 | + | 11832 | 0.66 | 0.858375 |
Target: 5'- uUGuAGGCAAC--GACCUGGAgCUGCUc -3' miRNA: 3'- gAC-UUCGUUGagUUGGACCU-GAUGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 6227 | 0.66 | 0.858375 |
Target: 5'- ---cGGCAcaACUCGACCgUGGGC-GCCUu -3' miRNA: 3'- gacuUCGU--UGAGUUGG-ACCUGaUGGA- -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 37030 | 0.66 | 0.849355 |
Target: 5'- uUGAccGGCA---CGACCUGGAcCUACCc -3' miRNA: 3'- gACU--UCGUugaGUUGGACCU-GAUGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 32757 | 0.66 | 0.849355 |
Target: 5'- gCUGggGCgGGCg-GAUCUGGgcGCUGCCg -3' miRNA: 3'- -GACuuCG-UUGagUUGGACC--UGAUGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 27972 | 0.66 | 0.849355 |
Target: 5'- --aAAGCAGCUCAGCgaguggCUGGAaaaggGCCUg -3' miRNA: 3'- gacUUCGUUGAGUUG------GACCUga---UGGA- -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 24598 | 0.66 | 0.849355 |
Target: 5'- uCUGAaagccGGCGACUUcgugAAgCUGGACgGCCa -3' miRNA: 3'- -GACU-----UCGUUGAG----UUgGACCUGaUGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 23111 | 0.66 | 0.840072 |
Target: 5'- -cGGcAGCAACUgagCAGCCUGGguGCUAUCc -3' miRNA: 3'- gaCU-UCGUUGA---GUUGGACC--UGAUGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 14028 | 0.67 | 0.820763 |
Target: 5'- gCUGGAGauccGCcgCGAgCUGGACUACUUg -3' miRNA: 3'- -GACUUCgu--UGa-GUUgGACCUGAUGGA- -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 37158 | 0.67 | 0.81076 |
Target: 5'- -cGAA-CGACaCGGCCUGGGCUuccACCUg -3' miRNA: 3'- gaCUUcGUUGaGUUGGACCUGA---UGGA- -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 27685 | 0.67 | 0.81076 |
Target: 5'- -cGAA-CGGCUUGGCCUGGAgCUGCUc -3' miRNA: 3'- gaCUUcGUUGAGUUGGACCU-GAUGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 17631 | 0.67 | 0.81076 |
Target: 5'- -gGAGGCAGCUC-ACCUGGcCggACg- -3' miRNA: 3'- gaCUUCGUUGAGuUGGACCuGa-UGga -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 38135 | 0.67 | 0.800542 |
Target: 5'- gUGggGUucCUCAACCaGuGCUGCCUu -3' miRNA: 3'- gACuuCGuuGAGUUGGaCcUGAUGGA- -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 22821 | 0.67 | 0.800542 |
Target: 5'- --aAGGCGACUCcGCCgcuucgGGACUgGCCa -3' miRNA: 3'- gacUUCGUUGAGuUGGa-----CCUGA-UGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 25718 | 0.67 | 0.79012 |
Target: 5'- gCUGGagcGGCAACUCcaguACCUGG--UGCCg -3' miRNA: 3'- -GACU---UCGUUGAGu---UGGACCugAUGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 26420 | 0.68 | 0.767635 |
Target: 5'- aCUGucGGCGGCUC-ACCUGcGGCUgagcaauACCUa -3' miRNA: 3'- -GACu-UCGUUGAGuUGGAC-CUGA-------UGGA- -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 8320 | 0.68 | 0.765455 |
Target: 5'- uCUGcauGCGGCUgAcgugauccgcauggGCCUGGGCUAUCUg -3' miRNA: 3'- -GACuu-CGUUGAgU--------------UGGACCUGAUGGA- -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 16495 | 0.68 | 0.735448 |
Target: 5'- cCUGGAGCAGCagcugCAGCgCgUGGGCUgggACCg -3' miRNA: 3'- -GACUUCGUUGa----GUUG-G-ACCUGA---UGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 2638 | 0.69 | 0.664873 |
Target: 5'- cCUGGAuacGCAcguacgccuugguGCUCAcgACCUGGACaGCCUc -3' miRNA: 3'- -GACUU---CGU-------------UGAGU--UGGACCUGaUGGA- -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 28770 | 0.7 | 0.654276 |
Target: 5'- -gGAAGCccguGCUCGcgccGCCUGGGCgACCc -3' miRNA: 3'- gaCUUCGu---UGAGU----UGGACCUGaUGGa -5' |
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30556 | 3' | -50.4 | NC_006548.1 | + | 637 | 0.7 | 0.642479 |
Target: 5'- -aGAacaGGCGGC-CGGCCUGGaacaGCUGCCg -3' miRNA: 3'- gaCU---UCGUUGaGUUGGACC----UGAUGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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