Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30557 | 3' | -54.3 | NC_006548.1 | + | 16483 | 0.71 | 0.320568 |
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Target: 5'- uGCGGCagagcaccuggagcAGCAGCUGCagcgcgugggcugGGACC-GCGUCa -3' miRNA: 3'- -CGCCG--------------UCGUUGACGa------------UCUGGaCGUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 37841 | 0.69 | 0.438074 |
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Target: 5'- gGUGGCuGCuccgAACgugGCUGGugCUGCAaaUCa -3' miRNA: 3'- -CGCCGuCG----UUGa--CGAUCugGACGU--AG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 33315 | 0.68 | 0.469242 |
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Target: 5'- uGCGGCGcugcGCAGcCUGCUGuacGcCCUGCGUg -3' miRNA: 3'- -CGCCGU----CGUU-GACGAU---CuGGACGUAg -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 35265 | 0.68 | 0.469242 |
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Target: 5'- cUGGCGGCGAUggugggcgGcCUGGuACCUGCGUUc -3' miRNA: 3'- cGCCGUCGUUGa-------C-GAUC-UGGACGUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 13576 | 0.68 | 0.4874 |
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Target: 5'- gGCGGCGGCaAGCUGCUgcucaaccagauccAGaaaaGCCUGgAUUc -3' miRNA: 3'- -CGCCGUCG-UUGACGA--------------UC----UGGACgUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 22916 | 0.68 | 0.501506 |
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Target: 5'- cGCGGCGGCGA--GUUGGGCCggacUAUCg -3' miRNA: 3'- -CGCCGUCGUUgaCGAUCUGGac--GUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 16343 | 0.68 | 0.512478 |
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Target: 5'- cGUGGCGGCAAC-----GACCUGCGg- -3' miRNA: 3'- -CGCCGUCGUUGacgauCUGGACGUag -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 7768 | 0.67 | 0.544821 |
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Target: 5'- cGCGGCAGCucaugcGCUcgaugucgucuagGUcgAGGCCUGCAa- -3' miRNA: 3'- -CGCCGUCGu-----UGA-------------CGa-UCUGGACGUag -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 29140 | 1.13 | 0.000322 |
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Target: 5'- cGCGGCAGCAACUGCUAGACCUGCAUCg -3' miRNA: 3'- -CGCCGUCGUUGACGAUCUGGACGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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