Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30557 | 3' | -54.3 | NC_006548.1 | + | 16483 | 0.71 | 0.320568 |
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Target: 5'- uGCGGCagagcaccuggagcAGCAGCUGCagcgcgugggcugGGACC-GCGUCa -3' miRNA: 3'- -CGCCG--------------UCGUUGACGa------------UCUGGaCGUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 14967 | 0.72 | 0.30996 |
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Target: 5'- -gGGUAGCAGCcGUgauGACCUGCAg- -3' miRNA: 3'- cgCCGUCGUUGaCGau-CUGGACGUag -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 30500 | 0.73 | 0.257443 |
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Target: 5'- cGCGGguGCAACUGCUGGcauggaACCaGCc-- -3' miRNA: 3'- -CGCCguCGUUGACGAUC------UGGaCGuag -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 7271 | 0.73 | 0.257443 |
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Target: 5'- cGCGGCAGa----GCUGGGCCUGUcgCu -3' miRNA: 3'- -CGCCGUCguugaCGAUCUGGACGuaG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 20581 | 0.73 | 0.250565 |
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Target: 5'- aGCGGCGGCAgagGCUGCU--GCg-GCGUCg -3' miRNA: 3'- -CGCCGUCGU---UGACGAucUGgaCGUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 21214 | 0.74 | 0.212434 |
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Target: 5'- cCGGUAGCAcugcGCUGCU-GAUCgGCAUCa -3' miRNA: 3'- cGCCGUCGU----UGACGAuCUGGaCGUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 35514 | 0.78 | 0.109349 |
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Target: 5'- cGUGGCAaggaucGCGGcCUGCUGGACCagGCGUCa -3' miRNA: 3'- -CGCCGU------CGUU-GACGAUCUGGa-CGUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 36367 | 0.83 | 0.046847 |
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Target: 5'- cUGGCuGCAGCUGCgccAGAUCUGCAUCg -3' miRNA: 3'- cGCCGuCGUUGACGa--UCUGGACGUAG- -5' |
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| 30557 | 3' | -54.3 | NC_006548.1 | + | 29140 | 1.13 | 0.000322 |
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Target: 5'- cGCGGCAGCAACUGCUAGACCUGCAUCg -3' miRNA: 3'- -CGCCGUCGUUGACGAUCUGGACGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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