Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30559 | 3' | -55.1 | NC_006548.1 | + | 27893 | 0.66 | 0.619669 |
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Target: 5'- gAGCAGGAUGCCAu--UGGUuuccuGGCGGGu -3' miRNA: 3'- -UCGUUCUGUGGUcguACCA-----CUGCCCu -5' |
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| 30559 | 3' | -55.1 | NC_006548.1 | + | 27102 | 0.66 | 0.608273 |
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Target: 5'- cAGCGAGGcCGCCAcCAUGGcccUGAUGcGGGc -3' miRNA: 3'- -UCGUUCU-GUGGUcGUACC---ACUGC-CCU- -5' |
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| 30559 | 3' | -55.1 | NC_006548.1 | + | 24884 | 0.66 | 0.574258 |
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Target: 5'- cAGCAGGAUguagcgcuCCGGCgGUGGUG-CGGuGAa -3' miRNA: 3'- -UCGUUCUGu-------GGUCG-UACCACuGCC-CU- -5' |
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| 30559 | 3' | -55.1 | NC_006548.1 | + | 22738 | 0.67 | 0.55182 |
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Target: 5'- uGCucGAuCGCCAGCGUGGcGGCGuccuGGAu -3' miRNA: 3'- uCGuuCU-GUGGUCGUACCaCUGC----CCU- -5' |
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| 30559 | 3' | -55.1 | NC_006548.1 | + | 1649 | 0.67 | 0.540699 |
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Target: 5'- cGGCugGGCACau-CGUGGUGAacaGGGGg -3' miRNA: 3'- -UCGuuCUGUGgucGUACCACUg--CCCU- -5' |
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| 30559 | 3' | -55.1 | NC_006548.1 | + | 23863 | 0.69 | 0.424861 |
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Target: 5'- cGCGc--CACCGGCAUGGccGGCGGGc -3' miRNA: 3'- uCGUucuGUGGUCGUACCa-CUGCCCu -5' |
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| 30559 | 3' | -55.1 | NC_006548.1 | + | 35941 | 0.72 | 0.257302 |
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Target: 5'- cGGCGAGuugGCCGGCAgGGUGGCGGc- -3' miRNA: 3'- -UCGUUCug-UGGUCGUaCCACUGCCcu -5' |
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| 30559 | 3' | -55.1 | NC_006548.1 | + | 29499 | 1.09 | 0.000548 |
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Target: 5'- gAGCAAGACACCAGCAUGGUGACGGGAg -3' miRNA: 3'- -UCGUUCUGUGGUCGUACCACUGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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