Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30559 | 5' | -59.4 | NC_006548.1 | + | 26375 | 0.66 | 0.361121 |
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Target: 5'- --aCGCgCUCUGCUGCAGGuaguaaaAGCGCg-- -3' miRNA: 3'- acaGUG-GAGGCGGCGUCC-------UCGCGaaa -5' |
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| 30559 | 5' | -59.4 | NC_006548.1 | + | 488 | 0.67 | 0.31786 |
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Target: 5'- -aUUACCUCCgcuugcuaucugcgGCCGCcuGGAGCGUUUc -3' miRNA: 3'- acAGUGGAGG--------------CGGCGu-CCUCGCGAAa -5' |
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| 30559 | 5' | -59.4 | NC_006548.1 | + | 33286 | 0.67 | 0.312343 |
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Target: 5'- cGUUggcagACCUCCgGCCGCgAGGAGCauGCg-- -3' miRNA: 3'- aCAG-----UGGAGG-CGGCG-UCCUCG--CGaaa -5' |
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| 30559 | 5' | -59.4 | NC_006548.1 | + | 24809 | 0.69 | 0.228943 |
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Target: 5'- aUGuUCACCgcaCCaCCGCcGGAGCGCUa- -3' miRNA: 3'- -AC-AGUGGa--GGcGGCGuCCUCGCGAaa -5' |
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| 30559 | 5' | -59.4 | NC_006548.1 | + | 7784 | 0.7 | 0.178935 |
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Target: 5'- ---gACCUCCGCgaggacaaggaagCGCAGGGGCGCc-- -3' miRNA: 3'- acagUGGAGGCG-------------GCGUCCUCGCGaaa -5' |
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| 30559 | 5' | -59.4 | NC_006548.1 | + | 32418 | 0.75 | 0.084315 |
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Target: 5'- cGUCGCCgCCguugccguucgcggcGCUGCAGGAGCGCa-- -3' miRNA: 3'- aCAGUGGaGG---------------CGGCGUCCUCGCGaaa -5' |
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| 30559 | 5' | -59.4 | NC_006548.1 | + | 13312 | 0.83 | 0.02063 |
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Target: 5'- gGUUugC-CCGCCGCAGGAGCGCg-- -3' miRNA: 3'- aCAGugGaGGCGGCGUCCUCGCGaaa -5' |
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| 30559 | 5' | -59.4 | NC_006548.1 | + | 29533 | 1.04 | 0.000425 |
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Target: 5'- cUGUCACCUCCGCCGCAGGAGCGCUUUc -3' miRNA: 3'- -ACAGUGGAGGCGGCGUCCUCGCGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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