Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30560 | 5' | -51.4 | NC_006548.1 | + | 18447 | 0.66 | 0.812563 |
Target: 5'- -gGGugG-CAGC-GGCGUGGUcuucugcaGGGCg -3' miRNA: 3'- gaCCugUaGUUGuUCGCACCAa-------CCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 9631 | 0.67 | 0.749649 |
Target: 5'- -gGGAuCAggucgauaugcUCGACAGGUGgcugGGUgcgGGGCa -3' miRNA: 3'- gaCCU-GU-----------AGUUGUUCGCa---CCAa--CCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 23822 | 0.68 | 0.71508 |
Target: 5'- cCUGGGCcagCAGCAcauaggcugcuGGCGUGcggaaaucucgcaGUUGGGUg -3' miRNA: 3'- -GACCUGua-GUUGU-----------UCGCAC-------------CAACCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 25370 | 0.69 | 0.670387 |
Target: 5'- cCUGGGCAaugagcugcgCGACcAGCGaaUGG-UGGGCg -3' miRNA: 3'- -GACCUGUa---------GUUGuUCGC--ACCaACCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 6536 | 0.69 | 0.658799 |
Target: 5'- cCUGGGCAUCGaggucaucguGCAcgcgccagggcGGCGcgGGUUacGGGCu -3' miRNA: 3'- -GACCUGUAGU----------UGU-----------UCGCa-CCAA--CCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 3991 | 0.69 | 0.657638 |
Target: 5'- uCUGG-CGUCAACAuccaucAGCGgGGUguacgcgUGGGUg -3' miRNA: 3'- -GACCuGUAGUUGU------UCGCaCCA-------ACCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 27957 | 0.69 | 0.651832 |
Target: 5'- cCUGGGCAccaacacaaagcagcUCAGCGAGUGgcUGGaaaaGGGCc -3' miRNA: 3'- -GACCUGU---------------AGUUGUUCGC--ACCaa--CCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 1150 | 0.7 | 0.577565 |
Target: 5'- uUGGGCGUCAGCGAugcugagcuGCGccGGcUGGGUc -3' miRNA: 3'- gACCUGUAGUUGUU---------CGCa-CCaACCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 34013 | 0.71 | 0.532011 |
Target: 5'- -gGGACAUCcacuggAACGAGCcccaggggcuuGUGGUguggaUGGGCa -3' miRNA: 3'- gaCCUGUAG------UUGUUCG-----------CACCA-----ACCCG- -5' |
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30560 | 5' | -51.4 | NC_006548.1 | + | 29861 | 1.13 | 0.000872 |
Target: 5'- cCUGGACAUCAACAAGCGUGGUUGGGCu -3' miRNA: 3'- -GACCUGUAGUUGUUCGCACCAACCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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