Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30561 | 5' | -56.9 | NC_006548.1 | + | 3962 | 0.66 | 0.491064 |
Target: 5'- cUUCAGGGCUuccCGGCaGuccUGCuCGCUCUGg -3' miRNA: 3'- -AAGUCUCGAu--GCCG-Cu--AUG-GCGGGAC- -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 17435 | 0.66 | 0.491064 |
Target: 5'- aUCAGccguccgacccAGCaGCGGCGAUggcGCCGCagCUGg -3' miRNA: 3'- aAGUC-----------UCGaUGCCGCUA---UGGCGg-GAC- -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 25273 | 0.66 | 0.491064 |
Target: 5'- aUCGGAucauCGGCGuccuUGCCGCCCa- -3' miRNA: 3'- aAGUCUcgauGCCGCu---AUGGCGGGac -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 22502 | 0.66 | 0.491064 |
Target: 5'- cUCcGAGggGCGGCcaGAgcgGCCGCCCa- -3' miRNA: 3'- aAGuCUCgaUGCCG--CUa--UGGCGGGac -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 29878 | 0.66 | 0.459763 |
Target: 5'- -gCuGGGUUGCGGCc--GCCGCCCg- -3' miRNA: 3'- aaGuCUCGAUGCCGcuaUGGCGGGac -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 11728 | 0.67 | 0.415862 |
Target: 5'- -cCGGGGUUGCGGCGAUGacaugacgaugcucCCGauaggguggccauCCCUGg -3' miRNA: 3'- aaGUCUCGAUGCCGCUAU--------------GGC-------------GGGAC- -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 21216 | 0.67 | 0.410076 |
Target: 5'- aUCAcGAuacgGCggGCGGCGAUGgCGCCgCUGg -3' miRNA: 3'- aAGU-CU----CGa-UGCCGCUAUgGCGG-GAC- -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 33308 | 0.67 | 0.409117 |
Target: 5'- --aGGAGCaUGCGGCGcUGCgcagccugcuguaCGCCCUGc -3' miRNA: 3'- aagUCUCG-AUGCCGCuAUG-------------GCGGGAC- -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 26357 | 0.68 | 0.381913 |
Target: 5'- -cCAGAGCUACGGa---ACCGCCa-- -3' miRNA: 3'- aaGUCUCGAUGCCgcuaUGGCGGgac -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 34453 | 0.68 | 0.363867 |
Target: 5'- --aGGAGCaACaGCGAggUGCCGCCCa- -3' miRNA: 3'- aagUCUCGaUGcCGCU--AUGGCGGGac -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 30438 | 0.7 | 0.268335 |
Target: 5'- gUUCAauGCUGgGGCGAUcCCGCCCg- -3' miRNA: 3'- -AAGUcuCGAUgCCGCUAuGGCGGGac -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 32654 | 0.7 | 0.268335 |
Target: 5'- gUC-GAGCaACGGCGAUcGCUGCgCCUGu -3' miRNA: 3'- aAGuCUCGaUGCCGCUA-UGGCG-GGAC- -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 17519 | 0.72 | 0.205087 |
Target: 5'- cUUCGGccAGCUGCGGCGccAUCGCCgCUGc -3' miRNA: 3'- -AAGUC--UCGAUGCCGCuaUGGCGG-GAC- -5' |
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30561 | 5' | -56.9 | NC_006548.1 | + | 30376 | 1.07 | 0.000512 |
Target: 5'- aUUCAGAGCUACGGCGAUACCGCCCUGg -3' miRNA: 3'- -AAGUCUCGAUGCCGCUAUGGCGGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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