Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30562 | 3' | -56.6 | NC_006548.1 | + | 24662 | 0.66 | 0.590123 |
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Target: 5'- -aGUGcuaccacGCCCGCGGAACUGGucuuGGGUaCGg -3' miRNA: 3'- agCAC-------UGGGCGCUUUGGCU----CCCA-GCg -5' |
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| 30562 | 3' | -56.6 | NC_006548.1 | + | 30856 | 0.66 | 0.57919 |
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Target: 5'- uUCGUuggcuGACCCaccugcgacaGUGAAACCGAgccagcgcccggcGGGaUCGCg -3' miRNA: 3'- -AGCA-----CUGGG----------CGCUUUGGCU-------------CCC-AGCG- -5' |
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| 30562 | 3' | -56.6 | NC_006548.1 | + | 24571 | 0.68 | 0.484687 |
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Target: 5'- -gGUGACCgGUGAAGuccUCGAGGG-CGa -3' miRNA: 3'- agCACUGGgCGCUUU---GGCUCCCaGCg -5' |
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| 30562 | 3' | -56.6 | NC_006548.1 | + | 17336 | 0.68 | 0.474503 |
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Target: 5'- ---cGACCCGCGGcuacguacaagaGACCuauGGGGUCGa -3' miRNA: 3'- agcaCUGGGCGCU------------UUGGc--UCCCAGCg -5' |
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| 30562 | 3' | -56.6 | NC_006548.1 | + | 11746 | 0.68 | 0.474503 |
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Target: 5'- gCGcGGCgCGCGGcggcGCCG-GGGUUGCg -3' miRNA: 3'- aGCaCUGgGCGCUu---UGGCuCCCAGCG- -5' |
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| 30562 | 3' | -56.6 | NC_006548.1 | + | 35937 | 0.69 | 0.379258 |
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Target: 5'- ---cGACCgGCGAGuugGCCGgcAGGGUgGCg -3' miRNA: 3'- agcaCUGGgCGCUU---UGGC--UCCCAgCG- -5' |
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| 30562 | 3' | -56.6 | NC_006548.1 | + | 30939 | 1.11 | 0.00043 |
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Target: 5'- cUCGUGACCCGCGAAACCGAGGGUCGCc -3' miRNA: 3'- -AGCACUGGGCGCUUUGGCUCCCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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