Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30562 | 5' | -53.2 | NC_006548.1 | + | 36832 | 0.66 | 0.751851 |
Target: 5'- -aGCGAg-UCGAcCGGCGCgaggcucUCUGCGa -3' miRNA: 3'- ccCGCUgaAGCU-GCCGCGaa-----AGAUGC- -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 35941 | 0.66 | 0.75185 |
Target: 5'- cGGCGAgUUggccggcagggUGGCGGCGCgcgaCUGCa -3' miRNA: 3'- cCCGCUgAA-----------GCUGCCGCGaaa-GAUGc -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 16552 | 0.66 | 0.737911 |
Target: 5'- cGGCGugUUCuACGGUuacgcgguauccgaGCUgaUCUACGg -3' miRNA: 3'- cCCGCugAAGcUGCCG--------------CGAa-AGAUGC- -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 18448 | 0.67 | 0.708382 |
Target: 5'- cGGGUGGCa---GCGGCGUggucUUCUGCa -3' miRNA: 3'- -CCCGCUGaagcUGCCGCGa---AAGAUGc -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 17956 | 0.67 | 0.708381 |
Target: 5'- aGGGUGGCcagccUGGCgGGCGCgagugaUCUACGa -3' miRNA: 3'- -CCCGCUGaa---GCUG-CCGCGaa----AGAUGC- -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 23775 | 0.68 | 0.663645 |
Target: 5'- uGGGUGAUcgugccaagUUCGGCGGCGCcgcCcACGu -3' miRNA: 3'- -CCCGCUG---------AAGCUGCCGCGaaaGaUGC- -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 26339 | 0.68 | 0.629722 |
Target: 5'- cGGCGGCcgggUCGAUGGCcagagCUACGg -3' miRNA: 3'- cCCGCUGa---AGCUGCCGcgaaaGAUGC- -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 36009 | 0.69 | 0.551109 |
Target: 5'- aGGuGCGGC-UCGAuCGGCGCgc-CUAUGc -3' miRNA: 3'- -CC-CGCUGaAGCU-GCCGCGaaaGAUGC- -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 11745 | 0.7 | 0.507475 |
Target: 5'- -cGCGGCgcgCGGCGGCGCcggggUUGCGg -3' miRNA: 3'- ccCGCUGaa-GCUGCCGCGaaa--GAUGC- -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 3334 | 0.71 | 0.486208 |
Target: 5'- cGGUGAUUUCGggguACGGCGUg--CUGCa -3' miRNA: 3'- cCCGCUGAAGC----UGCCGCGaaaGAUGc -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 13386 | 0.71 | 0.445013 |
Target: 5'- cGGCGGaaUCGGCGcGCGCUc-CUGCGg -3' miRNA: 3'- cCCGCUgaAGCUGC-CGCGAaaGAUGC- -5' |
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30562 | 5' | -53.2 | NC_006548.1 | + | 30973 | 1.11 | 0.000919 |
Target: 5'- uGGGCGACUUCGACGGCGCUUUCUACGu -3' miRNA: 3'- -CCCGCUGAAGCUGCCGCGAAAGAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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