Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30563 | 5' | -59 | NC_006548.1 | + | 35055 | 0.66 | 0.399749 |
Target: 5'- -cGGc-CGCCCAUUgcuCGCCuCCGCAc -3' miRNA: 3'- aaCCuuGCGGGUGAaguGCGG-GGCGU- -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 1542 | 0.66 | 0.39051 |
Target: 5'- aUGGAGCGCuaCCACUcCACuGCCggcgaCCGCc -3' miRNA: 3'- aACCUUGCG--GGUGAaGUG-CGG-----GGCGu -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 36214 | 0.66 | 0.381412 |
Target: 5'- cUGGAcccCGCCCGac-CGCaCCCCGCGg -3' miRNA: 3'- aACCUu--GCGGGUgaaGUGcGGGGCGU- -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 32822 | 0.66 | 0.381412 |
Target: 5'- -cGGcAGCGCCCAgaUCcgccCGCCCCaGCc -3' miRNA: 3'- aaCC-UUGCGGGUgaAGu---GCGGGG-CGu -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 19671 | 0.66 | 0.381412 |
Target: 5'- -cGGAGCGCgCGCUgcagauaGCGCucuaCCCGCu -3' miRNA: 3'- aaCCUUGCGgGUGAag-----UGCG----GGGCGu -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 21625 | 0.67 | 0.34646 |
Target: 5'- cUGGAAguacgaccuCGCCCaggGCUUCAcCGUCCCGgAa -3' miRNA: 3'- aACCUU---------GCGGG---UGAAGU-GCGGGGCgU- -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 9723 | 0.67 | 0.306083 |
Target: 5'- -aGGAACa-CCGC--CAUGCCCCGCAc -3' miRNA: 3'- aaCCUUGcgGGUGaaGUGCGGGGCGU- -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 29985 | 0.68 | 0.269411 |
Target: 5'- -gGGAAUGCUgGCUaugccgcccgUCGCGUgCCGCAc -3' miRNA: 3'- aaCCUUGCGGgUGA----------AGUGCGgGGCGU- -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 6384 | 0.69 | 0.249157 |
Target: 5'- gUGGcGCGCCCACUUCaAUGCgacggCCGUAc -3' miRNA: 3'- aACCuUGCGGGUGAAG-UGCGg----GGCGU- -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 33308 | 0.7 | 0.22653 |
Target: 5'- -aGGAGCaugcggcgcugcgcaGCCUGCUgUACGCCCUGCGu -3' miRNA: 3'- aaCCUUG---------------CGGGUGAaGUGCGGGGCGU- -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 21538 | 0.7 | 0.224127 |
Target: 5'- aUGGAGCGCUC-CUUCAgGCuaCCgGCAg -3' miRNA: 3'- aACCUUGCGGGuGAAGUgCG--GGgCGU- -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 17052 | 0.74 | 0.108792 |
Target: 5'- -cGGAGCGCCCgGCUUCcaGCaGCUCCGCc -3' miRNA: 3'- aaCCUUGCGGG-UGAAG--UG-CGGGGCGu -5' |
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30563 | 5' | -59 | NC_006548.1 | + | 32337 | 1.05 | 0.000405 |
Target: 5'- uUUGGAACGCCCACUUCACGCCCCGCAc -3' miRNA: 3'- -AACCUUGCGGGUGAAGUGCGGGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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