Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30565 | 3' | -61.8 | NC_006548.1 | + | 1351 | 0.66 | 0.308718 |
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Target: 5'- uCCGGCa-GCUCgCGCaucUUGUACCCgAc -3' miRNA: 3'- -GGCCGgcCGAGgGCG---AACAUGGGgUa -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 2418 | 0.66 | 0.286767 |
|
Target: 5'- gCCGGCCuGGCgggugcgCCuCGCUcaagUGUGCUgCCAUg -3' miRNA: 3'- -GGCCGG-CCGa------GG-GCGA----ACAUGG-GGUA- -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 5502 | 0.68 | 0.228309 |
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Target: 5'- gUCGGCCuGGgUCuuGCUg--ACCCCGg -3' miRNA: 3'- -GGCCGG-CCgAGggCGAacaUGGGGUa -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 8944 | 0.66 | 0.286767 |
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Target: 5'- aUGGCCaGGCgcucgacCCCGCgguUGUACCUCu- -3' miRNA: 3'- gGCCGG-CCGa------GGGCGa--ACAUGGGGua -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 19983 | 0.66 | 0.293945 |
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Target: 5'- uCCGGCgcucagcgaGGC-CCCGCUcGUcaucggccACCCCAa -3' miRNA: 3'- -GGCCGg--------CCGaGGGCGAaCA--------UGGGGUa -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 20789 | 0.66 | 0.316315 |
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Target: 5'- -aGGCCGGCcgCCUGCUgccGCgCCAg -3' miRNA: 3'- ggCCGGCCGa-GGGCGAacaUGgGGUa -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 23139 | 0.71 | 0.133849 |
|
Target: 5'- aCGGCC-GCUCCCGCUcGauUGCaCCCAa -3' miRNA: 3'- gGCCGGcCGAGGGCGAaC--AUG-GGGUa -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 23524 | 0.7 | 0.166339 |
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Target: 5'- uCgGGCCGGaugaCCCGCUg--ACCCCGc -3' miRNA: 3'- -GgCCGGCCga--GGGCGAacaUGGGGUa -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 32985 | 1.06 | 0.000232 |
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Target: 5'- gCCGGCCGGCUCCCGCUUGUACCCCAUu -3' miRNA: 3'- -GGCCGGCCGAGGGCGAACAUGGGGUA- -5' |
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| 30565 | 3' | -61.8 | NC_006548.1 | + | 33032 | 0.66 | 0.286767 |
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Target: 5'- gCCGGCCGGCcagCgCCGCUgcaccggGcGCgCCAg -3' miRNA: 3'- -GGCCGGCCGa--G-GGCGAa------CaUGgGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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