Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30565 | 5' | -57.5 | NC_006548.1 | + | 31934 | 0.66 | 0.516243 |
Target: 5'- cGACGGAACcgacACCuGGCGCgucuuCCCu- -3' miRNA: 3'- aCUGCCUUGu---UGG-CCGCGacu--GGGca -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 29410 | 0.66 | 0.516243 |
Target: 5'- aGGCGGu-CAGCCGauCGCUGGCCgaCGUu -3' miRNA: 3'- aCUGCCuuGUUGGCc-GCGACUGG--GCA- -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 12198 | 0.66 | 0.516243 |
Target: 5'- aGugGGGACugUCGGCGCcGAUCg-- -3' miRNA: 3'- aCugCCUUGuuGGCCGCGaCUGGgca -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 32249 | 0.66 | 0.505682 |
Target: 5'- aUGACGu--CAACCGGCuGgUGugCCGc -3' miRNA: 3'- -ACUGCcuuGUUGGCCG-CgACugGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 16985 | 0.66 | 0.504631 |
Target: 5'- aGGCGGAGCugcuggaAGCCgGGCGCUccggGACCg-- -3' miRNA: 3'- aCUGCCUUG-------UUGG-CCGCGA----CUGGgca -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 36894 | 0.66 | 0.484847 |
Target: 5'- cGugGGcgcaccGCAACCGGCuGCUGcaGgCCGUc -3' miRNA: 3'- aCugCCu-----UGUUGGCCG-CGAC--UgGGCA- -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 13326 | 0.66 | 0.463422 |
Target: 5'- ---aGGAGCGcgcgccgauuccGCCGGCGCucaaacagcgaccUGGCCCGc -3' miRNA: 3'- acugCCUUGU------------UGGCCGCG-------------ACUGGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 25854 | 0.67 | 0.444472 |
Target: 5'- gUGGCGGAugucACCGcCuGCUGGCCCGc -3' miRNA: 3'- -ACUGCCUugu-UGGCcG-CGACUGGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 12634 | 0.67 | 0.444472 |
Target: 5'- uUGGCGGccgAGCGcCCGGCGUUGaagcGCUCGa -3' miRNA: 3'- -ACUGCC---UUGUuGGCCGCGAC----UGGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 32948 | 0.67 | 0.434675 |
Target: 5'- cGGCGauuCAACUGGCGC--GCCCGg -3' miRNA: 3'- aCUGCcuuGUUGGCCGCGacUGGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 6171 | 0.68 | 0.406054 |
Target: 5'- gGACGuGAacguccACGACCGuGUGCUGAucaCCCGc -3' miRNA: 3'- aCUGC-CU------UGUUGGC-CGCGACU---GGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 20459 | 0.69 | 0.352508 |
Target: 5'- uUGugGGAugAGCUGGUc--GGCCCGUu -3' miRNA: 3'- -ACugCCUugUUGGCCGcgaCUGGGCA- -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 33138 | 0.69 | 0.327674 |
Target: 5'- --cCGGGGCuGGCCGGUGCUcgggcagcGGCCCGa -3' miRNA: 3'- acuGCCUUG-UUGGCCGCGA--------CUGGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 3670 | 0.69 | 0.327674 |
Target: 5'- aGGCGGug-AACUGGCGCUGGCUg-- -3' miRNA: 3'- aCUGCCuugUUGGCCGCGACUGGgca -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 21299 | 0.7 | 0.296613 |
Target: 5'- cGGCGGGAauuUCGGUGCUGAUgCCGa -3' miRNA: 3'- aCUGCCUUguuGGCCGCGACUG-GGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 28967 | 0.7 | 0.281961 |
Target: 5'- -cACGGGugAcACCgaaGGCGCUGGCCUGg -3' miRNA: 3'- acUGCCUugU-UGG---CCGCGACUGGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 33306 | 0.73 | 0.174992 |
Target: 5'- cGA-GGAGCAugCGGCGCUGcgcaGCCUGc -3' miRNA: 3'- aCUgCCUUGUugGCCGCGAC----UGGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 36696 | 0.74 | 0.161134 |
Target: 5'- cGcCGGccuCAugCGGCGCUGGCaCCGUa -3' miRNA: 3'- aCuGCCuu-GUugGCCGCGACUG-GGCA- -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 21516 | 0.74 | 0.152453 |
Target: 5'- cGGCaGAACGGCCGGgGCgauguagacgGACCCGa -3' miRNA: 3'- aCUGcCUUGUUGGCCgCGa---------CUGGGCa -5' |
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30565 | 5' | -57.5 | NC_006548.1 | + | 33025 | 1.07 | 0.000584 |
Target: 5'- uUGACGGAACAACCGGCGCUGACCCGUc -3' miRNA: 3'- -ACUGCCUUGUUGGCCGCGACUGGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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