Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30566 | 5' | -59.1 | NC_006548.1 | + | 1196 | 0.66 | 0.453681 |
Target: 5'- cGCACaggCGUUG-CUGGCCGcGCaGACGg -3' miRNA: 3'- cCGUG---GCAACaGGCCGGCuCG-CUGCa -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 32879 | 0.66 | 0.443952 |
Target: 5'- --gACCGUuugUGcgCCGGCCGcGGUGACGc -3' miRNA: 3'- ccgUGGCA---ACa-GGCCGGC-UCGCUGCa -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 35852 | 0.66 | 0.434342 |
Target: 5'- uGGUucGCCGUg--CC-GUCGAGCGAUGUg -3' miRNA: 3'- -CCG--UGGCAacaGGcCGGCUCGCUGCA- -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 26104 | 0.66 | 0.424853 |
Target: 5'- cGGCACUGUUcuUCCuacGGCCGGuGcCGGCGg -3' miRNA: 3'- -CCGUGGCAAc-AGG---CCGGCU-C-GCUGCa -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 28961 | 0.66 | 0.41178 |
Target: 5'- cGGCGCCGgccgccucgacgGUuuGGCCG-GUGGCc- -3' miRNA: 3'- -CCGUGGCaa----------CAggCCGGCuCGCUGca -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 27974 | 0.67 | 0.406254 |
Target: 5'- cGCGCCGggaacgcgCUGGCCGAGCuGCa- -3' miRNA: 3'- cCGUGGCaaca----GGCCGGCUCGcUGca -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 35941 | 0.67 | 0.397148 |
Target: 5'- cGGCGa-GUUGgCCGGCaGGGUGGCGg -3' miRNA: 3'- -CCGUggCAACaGGCCGgCUCGCUGCa -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 12832 | 0.67 | 0.370631 |
Target: 5'- cGGCGCgGUaaGUCCGGUguggcuCGGGCGAaaCGUa -3' miRNA: 3'- -CCGUGgCAa-CAGGCCG------GCUCGCU--GCA- -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 28331 | 0.67 | 0.370631 |
Target: 5'- cGGCGCgCGUgaacgGUCCcGCCG-GUGAUGa -3' miRNA: 3'- -CCGUG-GCAa----CAGGcCGGCuCGCUGCa -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 22302 | 0.68 | 0.329194 |
Target: 5'- uGGUGCCGUUGcgggugUCGGCCaGGCGAUc- -3' miRNA: 3'- -CCGUGGCAACa-----GGCCGGcUCGCUGca -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 7054 | 0.68 | 0.313604 |
Target: 5'- aGGCGCuucgCGUuaUGUCgGGCCGAGUGGa-- -3' miRNA: 3'- -CCGUG----GCA--ACAGgCCGGCUCGCUgca -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 21523 | 0.69 | 0.29858 |
Target: 5'- aGGCuACCGgcagaaCGGCCgGGGCGAUGUa -3' miRNA: 3'- -CCG-UGGCaacag-GCCGG-CUCGCUGCA- -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 35741 | 0.69 | 0.284119 |
Target: 5'- cGCAgCGUccagaGUCCGGUCGAGCGGg-- -3' miRNA: 3'- cCGUgGCAa----CAGGCCGGCUCGCUgca -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 33279 | 0.69 | 0.283411 |
Target: 5'- cGGCuuCCGUUGgcagaccUCCGGCCGcgaggagcauGCGGCGc -3' miRNA: 3'- -CCGu-GGCAAC-------AGGCCGGCu---------CGCUGCa -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 20102 | 0.7 | 0.263475 |
Target: 5'- uGGUGCCGgaGUUC-GCCGAgGCGGCGa -3' miRNA: 3'- -CCGUGGCaaCAGGcCGGCU-CGCUGCa -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 22728 | 0.7 | 0.244065 |
Target: 5'- aGGUGCuCGUg--CCGGCCG-GCGAUGa -3' miRNA: 3'- -CCGUG-GCAacaGGCCGGCuCGCUGCa -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 22242 | 0.7 | 0.237865 |
Target: 5'- aGGCGCgaaucagguuCGgguucUGccCCGGCCGAGCGAUGUu -3' miRNA: 3'- -CCGUG----------GCa----ACa-GGCCGGCUCGCUGCA- -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 36649 | 0.72 | 0.187831 |
Target: 5'- aGCACCGcgGUgCCGGCC--GCGGCGUu -3' miRNA: 3'- cCGUGGCaaCA-GGCCGGcuCGCUGCA- -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 37544 | 0.72 | 0.182883 |
Target: 5'- cGGCGCCGgcgGUUgGGCUGAGUGGacUGUg -3' miRNA: 3'- -CCGUGGCaa-CAGgCCGGCUCGCU--GCA- -5' |
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30566 | 5' | -59.1 | NC_006548.1 | + | 17462 | 0.73 | 0.155555 |
Target: 5'- uGGCGCCGcag-CUGGCCGAaGCGAUGc -3' miRNA: 3'- -CCGUGGCaacaGGCCGGCU-CGCUGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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