Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30568 | 3' | -54.2 | NC_006548.1 | + | 16692 | 0.66 | 0.647409 |
Target: 5'- -----aGCGGAAGCGCCGAcgguuGCgGAc -3' miRNA: 3'- ugaagaCGCUUUCGCGGCUu----CGgCUu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 12587 | 0.66 | 0.647409 |
Target: 5'- gGCcagCUG-GucGGCGCCGGAuGCCGAGg -3' miRNA: 3'- -UGaa-GACgCuuUCGCGGCUU-CGGCUU- -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 16244 | 0.66 | 0.635829 |
Target: 5'- -----cGCGcAccGCGCCGAAGCCGu- -3' miRNA: 3'- ugaagaCGC-UuuCGCGGCUUCGGCuu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 20583 | 0.66 | 0.612668 |
Target: 5'- uGCUUCcGCGucAGCGgCaGAGGCUGAc -3' miRNA: 3'- -UGAAGaCGCuuUCGCgG-CUUCGGCUu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 6945 | 0.66 | 0.612668 |
Target: 5'- -----aGCuauuGGCGCCGGAGCCGGu -3' miRNA: 3'- ugaagaCGcuu-UCGCGGCUUCGGCUu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 22779 | 0.66 | 0.60111 |
Target: 5'- cGCcUUUGCGGAu-CGCCGAcgaGGCCGGg -3' miRNA: 3'- -UGaAGACGCUUucGCGGCU---UCGGCUu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 9113 | 0.66 | 0.60111 |
Target: 5'- gGCUUCUggaGCGccguccaauGAAGCGCCGGAaCUGGAa -3' miRNA: 3'- -UGAAGA---CGC---------UUUCGCGGCUUcGGCUU- -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 13370 | 0.68 | 0.499549 |
Target: 5'- cGCUcCUGCGGcgggcaaaccGGCGCCGAGGgCGGu -3' miRNA: 3'- -UGAaGACGCUu---------UCGCGGCUUCgGCUu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 4967 | 0.68 | 0.499549 |
Target: 5'- gAUggCUGCGGuuGCGCCacugGAgcGGCCGAAa -3' miRNA: 3'- -UGaaGACGCUuuCGCGG----CU--UCGGCUU- -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 36813 | 0.69 | 0.467323 |
Target: 5'- gGCUcUCUGCGAgcGuCGCCG-AGCUGGu -3' miRNA: 3'- -UGA-AGACGCUuuC-GCGGCuUCGGCUu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 17451 | 0.7 | 0.387112 |
Target: 5'- aGCagCgGCGAuGGCGCCGcagcuGGCCGAAg -3' miRNA: 3'- -UGaaGaCGCUuUCGCGGCu----UCGGCUU- -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 2315 | 0.75 | 0.188763 |
Target: 5'- gGCUgc-GCGgcGGCGCCGGAGCUGAc -3' miRNA: 3'- -UGAagaCGCuuUCGCGGCUUCGGCUu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 20368 | 0.83 | 0.047952 |
Target: 5'- gACUUCUucgucgagcGCGAAGGCGCCGAacgGGCCGAc -3' miRNA: 3'- -UGAAGA---------CGCUUUCGCGGCU---UCGGCUu -5' |
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30568 | 3' | -54.2 | NC_006548.1 | + | 34614 | 1.06 | 0.001087 |
Target: 5'- uACUUCUGCGAAAGCGCCGAAGCCGAAa -3' miRNA: 3'- -UGAAGACGCUUUCGCGGCUUCGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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