Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30568 | 5' | -51.6 | NC_006548.1 | + | 27839 | 0.66 | 0.83294 |
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Target: 5'- gGC-GACgccUUCGAAGAUCgUgCGCAGg -3' miRNA: 3'- gCGaCUGa--AGGCUUCUAGaAgGCGUC- -5' |
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| 30568 | 5' | -51.6 | NC_006548.1 | + | 26341 | 0.67 | 0.771648 |
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Target: 5'- gCGCUGGaugucggucagcgUUUCCGGccGGGUCUcCUGCAGc -3' miRNA: 3'- -GCGACU-------------GAAGGCU--UCUAGAaGGCGUC- -5' |
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| 30568 | 5' | -51.6 | NC_006548.1 | + | 3430 | 0.68 | 0.706717 |
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Target: 5'- gGCUGAUgUUCUGGAGAUCgaCUGCu- -3' miRNA: 3'- gCGACUG-AAGGCUUCUAGaaGGCGuc -5' |
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| 30568 | 5' | -51.6 | NC_006548.1 | + | 30071 | 0.68 | 0.695358 |
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Target: 5'- aCGCUGuuuuucaggguGgUUCCG-AGGUUUUUCGCAGg -3' miRNA: 3'- -GCGAC-----------UgAAGGCuUCUAGAAGGCGUC- -5' |
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| 30568 | 5' | -51.6 | NC_006548.1 | + | 7012 | 0.68 | 0.672451 |
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Target: 5'- cCGCUGGCg--CGAAGAUCcucgaCCGCAu -3' miRNA: 3'- -GCGACUGaagGCUUCUAGaa---GGCGUc -5' |
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| 30568 | 5' | -51.6 | NC_006548.1 | + | 19141 | 0.7 | 0.56865 |
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Target: 5'- gCGCUGGgagaUCCGAGcGggCUUCUGCAGg -3' miRNA: 3'- -GCGACUga--AGGCUU-CuaGAAGGCGUC- -5' |
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| 30568 | 5' | -51.6 | NC_006548.1 | + | 7082 | 0.73 | 0.438074 |
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Target: 5'- uGCUGAUgcggUCGAGGAUCUUCgCGcCAGc -3' miRNA: 3'- gCGACUGaa--GGCUUCUAGAAG-GC-GUC- -5' |
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| 30568 | 5' | -51.6 | NC_006548.1 | + | 34648 | 1.1 | 0.001327 |
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Target: 5'- gCGCUGACUUCCGAAGAUCUUCCGCAGa -3' miRNA: 3'- -GCGACUGAAGGCUUCUAGAAGGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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