Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30569 | 5' | -55.6 | NC_006548.1 | + | 6695 | 0.66 | 0.587525 |
Target: 5'- uGUGAUGGGC-GCCGagagUGGCGAgaacuucugcacGGUGCu -3' miRNA: 3'- uCGUUACCCGcCGGC----AUCGUU------------UCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 38276 | 0.66 | 0.587525 |
Target: 5'- gAGC---GGGUGGCCGaUGGCGAcauAGuCGUc -3' miRNA: 3'- -UCGuuaCCCGCCGGC-AUCGUU---UC-GCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 33054 | 0.66 | 0.58413 |
Target: 5'- uGCgAAUGGGguacaagcgggagcCGGCCGgccAGCGccgcugcaccGGGCGCg -3' miRNA: 3'- uCG-UUACCC--------------GCCGGCa--UCGU----------UUCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 33869 | 0.66 | 0.576225 |
Target: 5'- cGCGc---GCGGCCGgugAGCGAcgauggacGGCGCa -3' miRNA: 3'- uCGUuaccCGCCGGCa--UCGUU--------UCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 18879 | 0.66 | 0.576225 |
Target: 5'- cGCugaucgauUGGGCGGaCGUGGcCAAGGaCGUc -3' miRNA: 3'- uCGuu------ACCCGCCgGCAUC-GUUUC-GCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 6904 | 0.66 | 0.576225 |
Target: 5'- uAGCuggGAUGGauGCaGGCgGUAGCugugcAGCGCg -3' miRNA: 3'- -UCG---UUACC--CG-CCGgCAUCGuu---UCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 27368 | 0.66 | 0.564975 |
Target: 5'- gAGCAuuuccGGGCgGGCCGUguugaGGcCAAugccuaugccAGCGCg -3' miRNA: 3'- -UCGUua---CCCG-CCGGCA-----UC-GUU----------UCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 7621 | 0.66 | 0.561611 |
Target: 5'- cGCGcUGGuCGGCCGUcaugguuuccaccuGGC--GGCGCg -3' miRNA: 3'- uCGUuACCcGCCGGCA--------------UCGuuUCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 33588 | 0.66 | 0.553783 |
Target: 5'- cGCGcUGGGCaccguuguccGGCCG-AGCGAcGuCGCa -3' miRNA: 3'- uCGUuACCCG----------CCGGCaUCGUUuC-GCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 30418 | 0.66 | 0.531607 |
Target: 5'- cGCcuGAUGGGCGGCaCGgcGUucaaugcuGGGGCGa -3' miRNA: 3'- uCG--UUACCCGCCG-GCauCG--------UUUCGCg -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 3862 | 0.67 | 0.520639 |
Target: 5'- uGCAGUGcauGGCGGUCuaccuGCAGgagcGGCGCg -3' miRNA: 3'- uCGUUAC---CCGCCGGcau--CGUU----UCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 1261 | 0.67 | 0.50976 |
Target: 5'- cGCGGUGcGGCaGGCU-UGGCuuuGAGCGUc -3' miRNA: 3'- uCGUUAC-CCG-CCGGcAUCGu--UUCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 27212 | 0.67 | 0.50976 |
Target: 5'- cAGCAGgcGGCGaCCG-AGCAAcGCGCu -3' miRNA: 3'- -UCGUUacCCGCcGGCaUCGUUuCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 32752 | 0.67 | 0.498978 |
Target: 5'- uGCAGgcugGGGCGGgCGgaucuGGGCGCu -3' miRNA: 3'- uCGUUa---CCCGCCgGCaucguUUCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 9724 | 0.67 | 0.488299 |
Target: 5'- cAGCuauuucGUGGGCuuGGCCGUAGUu-GGCu- -3' miRNA: 3'- -UCGu-----UACCCG--CCGGCAUCGuuUCGcg -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 19699 | 0.67 | 0.488299 |
Target: 5'- cGCGuguaGGGCcuuucaGGCgCGUucGCGGAGCGCg -3' miRNA: 3'- uCGUua--CCCG------CCG-GCAu-CGUUUCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 1972 | 0.67 | 0.477728 |
Target: 5'- aGGgGAUcGGGCaccaGGCCGUGGCucGGCu- -3' miRNA: 3'- -UCgUUA-CCCG----CCGGCAUCGuuUCGcg -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 32808 | 0.68 | 0.456934 |
Target: 5'- uGGCAAgcgucaccGCGGCCGgcGCAcaaacggucGAGUGCg -3' miRNA: 3'- -UCGUUacc-----CGCCGGCauCGU---------UUCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 20896 | 0.68 | 0.456934 |
Target: 5'- aAGCc---GGCGGCCGaGGUGcuGCGCa -3' miRNA: 3'- -UCGuuacCCGCCGGCaUCGUuuCGCG- -5' |
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30569 | 5' | -55.6 | NC_006548.1 | + | 27854 | 0.68 | 0.453857 |
Target: 5'- cGCAGUG-GUGGCCGaGGCGAcgccuucgaagaucGuGCGCa -3' miRNA: 3'- uCGUUACcCGCCGGCaUCGUU--------------U-CGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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