Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30570 | 5' | -52.3 | NC_006548.1 | + | 17555 | 0.65 | 0.802482 |
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Target: 5'- gUUGGGucguccaguugcCGACGAcAGGCuGCAUCGCu -3' miRNA: 3'- gAGCCCua----------GCUGCU-UCUGuCGUAGUG- -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 8782 | 0.66 | 0.754517 |
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Target: 5'- gUCGGGAugUCGAUGAggagcuuuccguGGGCGGCcgC-Ca -3' miRNA: 3'- gAGCCCU--AGCUGCU------------UCUGUCGuaGuG- -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 22360 | 0.67 | 0.731583 |
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Target: 5'- uUCGG-AUCGGCGAucgaaccGGACAcGCgAUCACc -3' miRNA: 3'- gAGCCcUAGCUGCU-------UCUGU-CG-UAGUG- -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 30689 | 0.69 | 0.618979 |
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Target: 5'- -aCGGGAacugCGACGGcGGACAGgGUgGCa -3' miRNA: 3'- gaGCCCUa---GCUGCU-UCUGUCgUAgUG- -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 5535 | 0.69 | 0.573183 |
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Target: 5'- gCUCGaGcGGUCGACcuGAGcccGGCGGCGUCGCu -3' miRNA: 3'- -GAGC-C-CUAGCUG--CUU---CUGUCGUAGUG- -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 21569 | 0.71 | 0.506229 |
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Target: 5'- uUCGGGAUCGAUGGGaaauGguGCAUUGCu -3' miRNA: 3'- gAGCCCUAGCUGCUUc---UguCGUAGUG- -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 27632 | 0.72 | 0.453103 |
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Target: 5'- -aCGGGGUCGGCGgcGAaGGCGagGCa -3' miRNA: 3'- gaGCCCUAGCUGCuuCUgUCGUagUG- -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 35181 | 0.72 | 0.432714 |
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Target: 5'- uUCGGGGgagacaaGGcCGAGGGCGGCAUCGu -3' miRNA: 3'- gAGCCCUag-----CU-GCUUCUGUCGUAGUg -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 30810 | 0.79 | 0.154087 |
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Target: 5'- -gCGGGAUCG-CGGAGAUAGCAgcugCGCu -3' miRNA: 3'- gaGCCCUAGCuGCUUCUGUCGUa---GUG- -5' |
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| 30570 | 5' | -52.3 | NC_006548.1 | + | 35765 | 1.11 | 0.000989 |
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Target: 5'- gCUCGGGAUCGACGAAGACAGCAUCACa -3' miRNA: 3'- -GAGCCCUAGCUGCUUCUGUCGUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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