Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30574 | 5' | -52.2 | NC_006548.1 | + | 14987 | 0.66 | 0.791916 |
Target: 5'- gGACUCGgcagCagUGCGcucaccGGCAGCAUAg-GCg -3' miRNA: 3'- aCUGAGCa---G--ACGC------UCGUCGUAUagCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 29896 | 0.66 | 0.781539 |
Target: 5'- aUGACUgGUCcgGCGcuguGCGGCA---CGCg -3' miRNA: 3'- -ACUGAgCAGa-CGCu---CGUCGUauaGCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 27073 | 0.66 | 0.781539 |
Target: 5'- gGGC-CGUCUGCuGAuCGGCGgacUCGCc -3' miRNA: 3'- aCUGaGCAGACG-CUcGUCGUau-AGCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 38266 | 0.66 | 0.770991 |
Target: 5'- -cGCUCuGUCUgaGCGGGUGGCcgAUgGCg -3' miRNA: 3'- acUGAG-CAGA--CGCUCGUCGuaUAgCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 37306 | 0.66 | 0.760283 |
Target: 5'- aUGACUU-UCUGa-GGCAGCcgGUCGUc -3' miRNA: 3'- -ACUGAGcAGACgcUCGUCGuaUAGCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 36787 | 0.67 | 0.727343 |
Target: 5'- cGACUCG-CUGCGAaugaacCAGCGccaggCGCg -3' miRNA: 3'- aCUGAGCaGACGCUc-----GUCGUaua--GCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 1126 | 0.67 | 0.693454 |
Target: 5'- cUGGC-CGUCUGCGcGGCcAGCAa--CGCc -3' miRNA: 3'- -ACUGaGCAGACGC-UCG-UCGUauaGCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 36814 | 0.68 | 0.670512 |
Target: 5'- aGGCUC-UCUGCGAGCGucGCcgAgcuggUGCg -3' miRNA: 3'- aCUGAGcAGACGCUCGU--CGuaUa----GCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 30858 | 0.68 | 0.647409 |
Target: 5'- cGGC-CGUUaUGCGGGCAGCAgugaaGCc -3' miRNA: 3'- aCUGaGCAG-ACGCUCGUCGUauag-CG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 34378 | 0.68 | 0.647409 |
Target: 5'- cGcCUgGUCcuUGUGGGCGGCAccUCGCu -3' miRNA: 3'- aCuGAgCAG--ACGCUCGUCGUauAGCG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 24624 | 0.68 | 0.63467 |
Target: 5'- gGACUucaccggucaccgUGUCgGCGGGCAGCucgAUCuGCg -3' miRNA: 3'- aCUGA-------------GCAGaCGCUCGUCGua-UAG-CG- -5' |
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30574 | 5' | -52.2 | NC_006548.1 | + | 36935 | 1.12 | 0.000722 |
Target: 5'- uUGACUCGUCUGCGAGCAGCAUAUCGCu -3' miRNA: 3'- -ACUGAGCAGACGCUCGUCGUAUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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