Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30576 | 5' | -57 | NC_006548.1 | + | 22670 | 0.66 | 0.513997 |
Target: 5'- cGGCAguUCGGCGAGCgGaacgauagcGCCGUcCUGg -3' miRNA: 3'- cCCGU--AGCCGUUCGaC---------UGGCGaGACa -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 18530 | 0.66 | 0.513997 |
Target: 5'- cGGGUccauGUCGGCAcGCUGucACCGCa---- -3' miRNA: 3'- -CCCG----UAGCCGUuCGAC--UGGCGagaca -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 37544 | 0.66 | 0.513997 |
Target: 5'- cGGCGcCGGCGguugGGCUGAgUGgaCUGUg -3' miRNA: 3'- cCCGUaGCCGU----UCGACUgGCgaGACA- -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 3966 | 0.66 | 0.482268 |
Target: 5'- aGGGCuuccCGGCAGucCUGcUCGCUCUGg -3' miRNA: 3'- -CCCGua--GCCGUUc-GACuGGCGAGACa -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 23255 | 0.67 | 0.431624 |
Target: 5'- aGGCAUCGaGCAGuucGCUgucGACCGCUUg-- -3' miRNA: 3'- cCCGUAGC-CGUU---CGA---CUGGCGAGaca -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 22291 | 0.68 | 0.393428 |
Target: 5'- cGGGUGUCGGcCAGGC-GAUCGCg--GUa -3' miRNA: 3'- -CCCGUAGCC-GUUCGaCUGGCGagaCA- -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 23352 | 0.68 | 0.384227 |
Target: 5'- uGGGCAUC-----GCUGACCGCgCUGUu -3' miRNA: 3'- -CCCGUAGccguuCGACUGGCGaGACA- -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 26973 | 0.68 | 0.384227 |
Target: 5'- aGGC--CGaGCAAGCUGGCCGUaagCUGa -3' miRNA: 3'- cCCGuaGC-CGUUCGACUGGCGa--GACa -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 22565 | 0.68 | 0.384227 |
Target: 5'- uGGUG-CGGCAGGUgcaGCCGCUUUGUc -3' miRNA: 3'- cCCGUaGCCGUUCGac-UGGCGAGACA- -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 3654 | 0.68 | 0.37517 |
Target: 5'- gGGGCugaagccuuccuGUCGGaucugccGCUG-CCGCUCUGUc -3' miRNA: 3'- -CCCG------------UAGCCguu----CGACuGGCGAGACA- -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 20978 | 0.68 | 0.360982 |
Target: 5'- uGGGCAguUCGGCcagcAAGCUGcgcagcaccucggccGCCGgCUUUGUg -3' miRNA: 3'- -CCCGU--AGCCG----UUCGAC---------------UGGC-GAGACA- -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 33814 | 0.68 | 0.357494 |
Target: 5'- uGGcCAUCGGCGA-CcGACCGCUCg-- -3' miRNA: 3'- cCC-GUAGCCGUUcGaCUGGCGAGaca -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 29479 | 0.69 | 0.315909 |
Target: 5'- cGGCAacgUCGGCcagcgaucGCUGACCGC-CUGc -3' miRNA: 3'- cCCGU---AGCCGuu------CGACUGGCGaGACa -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 30575 | 0.7 | 0.301092 |
Target: 5'- aGGGCAUCGGCugguuccaugccagcAGUUGcacCCGCgggCUGUa -3' miRNA: 3'- -CCCGUAGCCGu--------------UCGACu--GGCGa--GACA- -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 20520 | 0.7 | 0.292761 |
Target: 5'- uGGGCAcUGGCAccguGGgUGACUGCUCg-- -3' miRNA: 3'- -CCCGUaGCCGU----UCgACUGGCGAGaca -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 1149 | 0.7 | 0.278079 |
Target: 5'- uGGGCGUCaGCGAuGCUGAgCUGCgccggCUGg -3' miRNA: 3'- -CCCGUAGcCGUU-CGACU-GGCGa----GACa -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 15809 | 0.7 | 0.27096 |
Target: 5'- uGGGUAUCGGCuGGGCgGACgaCGCUCa-- -3' miRNA: 3'- -CCCGUAGCCG-UUCGaCUG--GCGAGaca -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 15421 | 0.72 | 0.219213 |
Target: 5'- aGGGCAUCcuGCAGGgUGACCGUgugcaggCUGa -3' miRNA: 3'- -CCCGUAGc-CGUUCgACUGGCGa------GACa -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 4785 | 0.72 | 0.202094 |
Target: 5'- uGGCgAUCGGCAGGCUGugUGC-CUc- -3' miRNA: 3'- cCCG-UAGCCGUUCGACugGCGaGAca -5' |
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30576 | 5' | -57 | NC_006548.1 | + | 18042 | 0.73 | 0.181084 |
Target: 5'- cGGuCAagGGCGAGC-GACCGUUCUGg -3' miRNA: 3'- cCC-GUagCCGUUCGaCUGGCGAGACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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