Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30577 | 3' | -53.6 | NC_006549.1 | + | 90012 | 0.66 | 0.957194 |
Target: 5'- -aGGAGGCUcaUUUCUg---GCCCGAGg -3' miRNA: 3'- caCCUUCGA--AGAGAauggCGGGCUCg -5' |
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30577 | 3' | -53.6 | NC_006549.1 | + | 129651 | 0.66 | 0.953225 |
Target: 5'- uGUGGugcAGGCUUC-CUU-CCGUgaCGAGCg -3' miRNA: 3'- -CACC---UUCGAAGaGAAuGGCGg-GCUCG- -5' |
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30577 | 3' | -53.6 | NC_006549.1 | + | 71109 | 0.66 | 0.93986 |
Target: 5'- -aGGAGGCggaUCUUACCaaauaccugacGCCCGccauAGCu -3' miRNA: 3'- caCCUUCGaagAGAAUGG-----------CGGGC----UCG- -5' |
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30577 | 3' | -53.6 | NC_006549.1 | + | 56685 | 0.68 | 0.885594 |
Target: 5'- -aGGggGCUUCcuccgcgggaaagUCggggACCGCUaCGAGCc -3' miRNA: 3'- caCCuuCGAAG-------------AGaa--UGGCGG-GCUCG- -5' |
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30577 | 3' | -53.6 | NC_006549.1 | + | 36559 | 0.7 | 0.80491 |
Target: 5'- -cGGAAGCUUCUgaauacGCCGCCUGuccuucgaGGCg -3' miRNA: 3'- caCCUUCGAAGAgaa---UGGCGGGC--------UCG- -5' |
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30577 | 3' | -53.6 | NC_006549.1 | + | 1961 | 1.12 | 0.002504 |
Target: 5'- uGUGGAAGCUUCUCUUACCGCCCGAGCg -3' miRNA: 3'- -CACCUUCGAAGAGAAUGGCGGGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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