Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30577 | 5' | -49.3 | NC_006549.1 | + | 15571 | 0.66 | 0.997222 |
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Target: 5'- gGCGCCUAuGAgAGg--GUCUUUAC-CCu -3' miRNA: 3'- -UGCGGGU-CUgUCaaaUAGAGAUGaGG- -5' |
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| 30577 | 5' | -49.3 | NC_006549.1 | + | 32571 | 0.68 | 0.990494 |
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Target: 5'- uGCGCCgAGACGGUUUGUUac--CUCg -3' miRNA: 3'- -UGCGGgUCUGUCAAAUAGagauGAGg -5' |
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| 30577 | 5' | -49.3 | NC_006549.1 | + | 123943 | 0.68 | 0.984848 |
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Target: 5'- uACGCCCAcgcGGCaucguuuucgguuuuAGUUaGUCUUUugUCCa -3' miRNA: 3'- -UGCGGGU---CUG---------------UCAAaUAGAGAugAGG- -5' |
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| 30577 | 5' | -49.3 | NC_006549.1 | + | 114437 | 0.7 | 0.954447 |
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Target: 5'- uACGCucuccucgCCAGACcGgagUUGUCUCUGCgcgCCa -3' miRNA: 3'- -UGCG--------GGUCUGuCa--AAUAGAGAUGa--GG- -5' |
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| 30577 | 5' | -49.3 | NC_006549.1 | + | 16888 | 0.74 | 0.838393 |
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Target: 5'- uCGCCacgGGGCAGUcUGUCUCguacGCUCCc -3' miRNA: 3'- uGCGGg--UCUGUCAaAUAGAGa---UGAGG- -5' |
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| 30577 | 5' | -49.3 | NC_006549.1 | + | 1927 | 1.12 | 0.007565 |
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Target: 5'- gACGCCCAGACAGUUUAUCUCUACUCCc -3' miRNA: 3'- -UGCGGGUCUGUCAAAUAGAGAUGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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