Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30578 | 3' | -49.2 | NC_006549.1 | + | 53502 | 0.66 | 0.998858 |
Target: 5'- ---gCAGACA-GACgACGGAa-AACCCu -3' miRNA: 3'- guuaGUCUGUaCUG-UGCCUgcUUGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 120668 | 0.66 | 0.998858 |
Target: 5'- gCAuUCGGcCAUGAUgcuaGCGGcaugcuucGCGGGCCCc -3' miRNA: 3'- -GUuAGUCuGUACUG----UGCC--------UGCUUGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 118457 | 0.66 | 0.998614 |
Target: 5'- aCAAUCcaugaAGACc-GACAUGGGCGGuACCa -3' miRNA: 3'- -GUUAG-----UCUGuaCUGUGCCUGCU-UGGg -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 48189 | 0.66 | 0.998327 |
Target: 5'- ----aAGACGcucaACGCGGACGGuaaACCCa -3' miRNA: 3'- guuagUCUGUac--UGUGCCUGCU---UGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 98917 | 0.66 | 0.998327 |
Target: 5'- uGGUCAGGCucuugGACGCGca-GGAUCCa -3' miRNA: 3'- gUUAGUCUGua---CUGUGCcugCUUGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 83309 | 0.67 | 0.996627 |
Target: 5'- -----uGACGUGG-ACGGccaGCGAACCCg -3' miRNA: 3'- guuaguCUGUACUgUGCC---UGCUUGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 12580 | 0.67 | 0.995967 |
Target: 5'- uCAAUCAaACGUcaucgccucgguaGACAUGGGCGAACa- -3' miRNA: 3'- -GUUAGUcUGUA-------------CUGUGCCUGCUUGgg -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 33299 | 0.67 | 0.994578 |
Target: 5'- aCGAgCAG-CGUGACgacgGCGGACGGAgUCg -3' miRNA: 3'- -GUUaGUCuGUACUG----UGCCUGCUUgGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 7097 | 0.68 | 0.991623 |
Target: 5'- cCAGUCGGGCuccagGGgucCGCGGGucccacCGGACCCg -3' miRNA: 3'- -GUUAGUCUGua---CU---GUGCCU------GCUUGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 48140 | 0.68 | 0.991506 |
Target: 5'- ----gGGACAUGGCACugcccgaaagcugGGGCcGGCCCa -3' miRNA: 3'- guuagUCUGUACUGUG-------------CCUGcUUGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 96786 | 0.69 | 0.985849 |
Target: 5'- --cUCAGAgGUGuuACuCGGACGGagaACCCg -3' miRNA: 3'- guuAGUCUgUAC--UGuGCCUGCU---UGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 47527 | 0.7 | 0.974846 |
Target: 5'- aAAUCAGAUAUGGUACagguucGACGGACUCa -3' miRNA: 3'- gUUAGUCUGUACUGUGc-----CUGCUUGGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 15373 | 0.71 | 0.947851 |
Target: 5'- uGAUCAGACAUGucggucugugcccucACGgccuguauagUGGACGAugCCa -3' miRNA: 3'- gUUAGUCUGUAC---------------UGU----------GCCUGCUugGG- -5' |
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30578 | 3' | -49.2 | NC_006549.1 | + | 3967 | 1.13 | 0.007795 |
Target: 5'- cCAAUCAGACAUGACACGGACGAACCCu -3' miRNA: 3'- -GUUAGUCUGUACUGUGCCUGCUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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