Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30578 | 5' | -56.9 | NC_006549.1 | + | 135086 | 0.66 | 0.877233 |
Target: 5'- -gGAGCUCGCCUACg-CGcAGCUg-- -3' miRNA: 3'- ggCUCGAGCGGAUGagGCaUCGGugc -5' |
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30578 | 5' | -56.9 | NC_006549.1 | + | 85078 | 0.66 | 0.862644 |
Target: 5'- gCCuAGCUCuGCCUACUCCGgcaa-ACGa -3' miRNA: 3'- -GGcUCGAG-CGGAUGAGGCaucggUGC- -5' |
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30578 | 5' | -56.9 | NC_006549.1 | + | 18832 | 0.66 | 0.855042 |
Target: 5'- aCCGuAGUggacgCGUCaUGCaCCGUAGUCACGg -3' miRNA: 3'- -GGC-UCGa----GCGG-AUGaGGCAUCGGUGC- -5' |
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30578 | 5' | -56.9 | NC_006549.1 | + | 134751 | 0.68 | 0.787708 |
Target: 5'- gCCGAGCggggugCGCCa--UCCGUaguuAGCCAUc -3' miRNA: 3'- -GGCUCGa-----GCGGaugAGGCA----UCGGUGc -5' |
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30578 | 5' | -56.9 | NC_006549.1 | + | 106320 | 0.7 | 0.6617 |
Target: 5'- gCGAacGCgugaCGCCaGCUCCGUGGCCGu- -3' miRNA: 3'- gGCU--CGa---GCGGaUGAGGCAUCGGUgc -5' |
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30578 | 5' | -56.9 | NC_006549.1 | + | 52627 | 0.74 | 0.455682 |
Target: 5'- -gGAGCUCGCCga-UCCGUccguGCCGCu -3' miRNA: 3'- ggCUCGAGCGGaugAGGCAu---CGGUGc -5' |
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30578 | 5' | -56.9 | NC_006549.1 | + | 96703 | 0.76 | 0.361995 |
Target: 5'- uCCGAGCUCGUCU-CUCCcGUGuCCACa -3' miRNA: 3'- -GGCUCGAGCGGAuGAGG-CAUcGGUGc -5' |
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30578 | 5' | -56.9 | NC_006549.1 | + | 3931 | 1.11 | 0.001733 |
Target: 5'- cCCGAGCUCGCCUACUCCGUAGCCACGg -3' miRNA: 3'- -GGCUCGAGCGGAUGAGGCAUCGGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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