Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30579 | 5' | -51.9 | NC_006549.1 | + | 109155 | 0.66 | 0.987499 |
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Target: 5'- gCACGCCGUcguACGUaAGACCcuuUCGc -3' miRNA: 3'- -GUGCGGCAu--UGCGcUCUGGaauAGCa -5' |
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| 30579 | 5' | -51.9 | NC_006549.1 | + | 33205 | 0.66 | 0.984049 |
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Target: 5'- -cCGCCGUAaaaACGgGAGAUUcUAUUGUa -3' miRNA: 3'- guGCGGCAU---UGCgCUCUGGaAUAGCA- -5' |
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| 30579 | 5' | -51.9 | NC_006549.1 | + | 92144 | 0.66 | 0.979922 |
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Target: 5'- gACGCCaUAGCguGCGGGGCC-UAUCa- -3' miRNA: 3'- gUGCGGcAUUG--CGCUCUGGaAUAGca -5' |
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| 30579 | 5' | -51.9 | NC_006549.1 | + | 92860 | 0.67 | 0.975048 |
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Target: 5'- -gUGCCG-AACGCGAGGCCg------ -3' miRNA: 3'- guGCGGCaUUGCGCUCUGGaauagca -5' |
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| 30579 | 5' | -51.9 | NC_006549.1 | + | 14561 | 0.67 | 0.975048 |
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Target: 5'- --aGCCGUGGCGCGAuGAuuuUCUUcgCGa -3' miRNA: 3'- gugCGGCAUUGCGCU-CU---GGAAuaGCa -5' |
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| 30579 | 5' | -51.9 | NC_006549.1 | + | 60443 | 0.67 | 0.975048 |
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Target: 5'- uGCGCCGaUAGCGUGGGGgCUc-UCGa -3' miRNA: 3'- gUGCGGC-AUUGCGCUCUgGAauAGCa -5' |
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| 30579 | 5' | -51.9 | NC_006549.1 | + | 4142 | 1.07 | 0.009569 |
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Target: 5'- gCACGCCGUAACGCGAGACCUUAUCGUc -3' miRNA: 3'- -GUGCGGCAUUGCGCUCUGGAAUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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