Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30580 | 3' | -56.8 | NC_006549.1 | + | 68262 | 0.66 | 0.855873 |
Target: 5'- gCGug-UCgGGUCCGGUGGacGCCCUUAc -3' miRNA: 3'- -GCuuaAGaUCGGGCCACC--CGGGAGUc -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 41829 | 0.66 | 0.848 |
Target: 5'- cCGGGggCgccgGGCCCGaGaGGGCCCgCGGg -3' miRNA: 3'- -GCUUaaGa---UCGGGC-CaCCCGGGaGUC- -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 50910 | 0.66 | 0.848 |
Target: 5'- ----gUCUGGCCCaGGcguuccugUGGGUCCUCuGg -3' miRNA: 3'- gcuuaAGAUCGGG-CC--------ACCCGGGAGuC- -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 55976 | 0.66 | 0.839931 |
Target: 5'- aCGuAcgUC-AGCCCGGUGucuggcugcgcGGCCUUUAGg -3' miRNA: 3'- -GC-UuaAGaUCGGGCCAC-----------CCGGGAGUC- -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 101926 | 0.66 | 0.839931 |
Target: 5'- -----cCUGGagaCCCGG-GGGCCCUgCAGg -3' miRNA: 3'- gcuuaaGAUC---GGGCCaCCCGGGA-GUC- -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 50854 | 0.67 | 0.787817 |
Target: 5'- -------cGGaCCCaGGUGGGCCCUUGGg -3' miRNA: 3'- gcuuaagaUC-GGG-CCACCCGGGAGUC- -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 50376 | 0.68 | 0.740461 |
Target: 5'- ----aUCUGGCCCGGgaggacccucUGGGCCgUCc- -3' miRNA: 3'- gcuuaAGAUCGGGCC----------ACCCGGgAGuc -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 49876 | 0.7 | 0.650026 |
Target: 5'- aCGGAccCUuggGGCCCGG-GGGCCCagGGg -3' miRNA: 3'- -GCUUaaGA---UCGGGCCaCCCGGGagUC- -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 81054 | 0.72 | 0.528447 |
Target: 5'- cCGAGaaaaUCguuGCCCGGUGGGCCgUCc- -3' miRNA: 3'- -GCUUa---AGau-CGGGCCACCCGGgAGuc -5' |
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30580 | 3' | -56.8 | NC_006549.1 | + | 7541 | 1.1 | 0.001889 |
Target: 5'- aCGAAUUCUAGCCCGGUGGGCCCUCAGg -3' miRNA: 3'- -GCUUAAGAUCGGGCCACCCGGGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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