Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30580 | 5' | -57.4 | NC_006549.1 | + | 7853 | 0.66 | 0.854713 |
Target: 5'- gGACCGGacGGuCCCGUAGGu-UCGAGGGg -3' miRNA: 3'- -CUGGUU--CCuGGGUAUCCccGGUUCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 16260 | 0.66 | 0.846839 |
Target: 5'- gGGCUAGGaGGCgUCGgAGGGGUgGGGGGa -3' miRNA: 3'- -CUGGUUC-CUG-GGUaUCCCCGgUUCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 50578 | 0.66 | 0.830516 |
Target: 5'- aACCcuGAGGACCCGUu-GGGCCAAc-- -3' miRNA: 3'- cUGG--UUCCUGGGUAucCCCGGUUccc -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 102389 | 0.66 | 0.822082 |
Target: 5'- uGGCCcuuguGGACCCAU-GGGGCCc---- -3' miRNA: 3'- -CUGGuu---CCUGGGUAuCCCCGGuuccc -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 102555 | 0.66 | 0.813476 |
Target: 5'- aGCCcuuuGGGCCCGc-GGGGCCGGcGGa -3' miRNA: 3'- cUGGuu--CCUGGGUauCCCCGGUUcCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 50489 | 0.67 | 0.804706 |
Target: 5'- gGGCCAucuGGcCCCGc-GGGGCCAAcgaggccaauGGGa -3' miRNA: 3'- -CUGGUu--CCuGGGUauCCCCGGUU----------CCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 49935 | 0.67 | 0.804706 |
Target: 5'- aGGCUggGGGCCCGcgucgccuuuGucGCCGAGGGg -3' miRNA: 3'- -CUGGuuCCUGGGUau--------CccCGGUUCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 49726 | 0.67 | 0.78671 |
Target: 5'- -cCCGcGGGCCCGU-GGGGCCcguuggcccgcGAGGu -3' miRNA: 3'- cuGGUuCCUGGGUAuCCCCGG-----------UUCCc -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 6965 | 0.67 | 0.76816 |
Target: 5'- gGGCCucAGGGCCCAgAGGGuCCugcGGGu -3' miRNA: 3'- -CUGGu-UCCUGGGUaUCCCcGGuu-CCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 100216 | 0.67 | 0.76816 |
Target: 5'- uGGCUggGGACCuCGggcuucgggggAGGGGUgGAGGa -3' miRNA: 3'- -CUGGuuCCUGG-GUa----------UCCCCGgUUCCc -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 59277 | 0.68 | 0.739455 |
Target: 5'- uACCAgAGGGCUCcUGGGuGGCguGGGGc -3' miRNA: 3'- cUGGU-UCCUGGGuAUCC-CCGguUCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 41144 | 0.68 | 0.729689 |
Target: 5'- -cCCAcGGGACCU--AGGGGcCCGAGaGGa -3' miRNA: 3'- cuGGU-UCCUGGGuaUCCCC-GGUUC-CC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 50859 | 0.68 | 0.699922 |
Target: 5'- -cCCAAcGGACCCA-GGuGGGCCcuuGGGu -3' miRNA: 3'- cuGGUU-CCUGGGUaUC-CCCGGuu-CCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 7109 | 0.7 | 0.639095 |
Target: 5'- gGGCCcauGGGCCCAgucGGGcuCCAGGGGu -3' miRNA: 3'- -CUGGuu-CCUGGGUau-CCCc-GGUUCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 119635 | 0.7 | 0.608494 |
Target: 5'- uGCCGcuGGGAgCU--GGGGGCgGAGGGg -3' miRNA: 3'- cUGGU--UCCUgGGuaUCCCCGgUUCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 14319 | 0.71 | 0.557934 |
Target: 5'- --gUAAGGAuaCCCGUAGGGGUgGAGGcGg -3' miRNA: 3'- cugGUUCCU--GGGUAUCCCCGgUUCC-C- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 41845 | 0.71 | 0.557934 |
Target: 5'- gGGCCcgcGAGGACCCccgGGGGcGCCGggcccgagAGGGc -3' miRNA: 3'- -CUGG---UUCCUGGGua-UCCC-CGGU--------UCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 7171 | 0.71 | 0.54795 |
Target: 5'- -cCCGAGGACCUcccGGGcCCAAGGGc -3' miRNA: 3'- cuGGUUCCUGGGuauCCCcGGUUCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 51016 | 0.72 | 0.51836 |
Target: 5'- aGCCuuGAGGGCCCc--GGGGCCccuuAGGGc -3' miRNA: 3'- cUGG--UUCCUGGGuauCCCCGGu---UCCC- -5' |
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30580 | 5' | -57.4 | NC_006549.1 | + | 82552 | 0.72 | 0.51836 |
Target: 5'- aGCCuuuAGGACCCAU-GGGGCCcguuGGc -3' miRNA: 3'- cUGGu--UCCUGGGUAuCCCCGGuu--CCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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