Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30581 | 3' | -57.6 | NC_006549.1 | + | 49725 | 0.66 | 0.864488 |
Target: 5'- cCGCGgGC-CCGUgGggcccguuggcccgcGAGGUCCCGCUu -3' miRNA: 3'- aGCGCgUGcGGCAgU---------------CUCUAGGGUGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 41829 | 0.66 | 0.853896 |
Target: 5'- cCGgGgGCGCCGggcccgAGAGggCCCGCg -3' miRNA: 3'- aGCgCgUGCGGCag----UCUCuaGGGUGg -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 73168 | 0.66 | 0.853896 |
Target: 5'- aUUGgGCAauCGCCGUucCAGcAGGUCCUGCg -3' miRNA: 3'- -AGCgCGU--GCGGCA--GUC-UCUAGGGUGg -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 6110 | 0.66 | 0.853896 |
Target: 5'- aCGgGCACGCCGUC-----UUCCAUCu -3' miRNA: 3'- aGCgCGUGCGGCAGucucuAGGGUGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 116461 | 0.67 | 0.82159 |
Target: 5'- -gGCGCuuGCCcaugGUCgccGGAGAUUCUGCCg -3' miRNA: 3'- agCGCGugCGG----CAG---UCUCUAGGGUGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 49924 | 0.67 | 0.795581 |
Target: 5'- cCGCGU-CGCCuuuGUCgccgAGGGGUCCCAUg -3' miRNA: 3'- aGCGCGuGCGG---CAG----UCUCUAGGGUGg -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 54889 | 0.68 | 0.768284 |
Target: 5'- aCGCGgG-GCaCGUCAGGGAcUCUCAUCa -3' miRNA: 3'- aGCGCgUgCG-GCAGUCUCU-AGGGUGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 22856 | 0.68 | 0.758938 |
Target: 5'- cUCGCGCGCGCCGuauacguuUCAGAuGGguuUCCUuuACg -3' miRNA: 3'- -AGCGCGUGCGGC--------AGUCU-CU---AGGG--UGg -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 59754 | 0.68 | 0.758938 |
Target: 5'- cCGUGUAUGCUGUCGGGGA-CgUACa -3' miRNA: 3'- aGCGCGUGCGGCAGUCUCUaGgGUGg -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 22612 | 0.68 | 0.758938 |
Target: 5'- uUCGCGUACGCaauGcUAGAGccccgcAUUCCGCCg -3' miRNA: 3'- -AGCGCGUGCGg--CaGUCUC------UAGGGUGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 30804 | 0.69 | 0.71075 |
Target: 5'- gUCGUGCGCGCgCGaguGGGucaaCCCGCCg -3' miRNA: 3'- -AGCGCGUGCG-GCaguCUCua--GGGUGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 84463 | 0.69 | 0.690959 |
Target: 5'- aCGCGCugGUC-UUAGAGAgaCCCuCCa -3' miRNA: 3'- aGCGCGugCGGcAGUCUCUa-GGGuGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 113847 | 0.7 | 0.630712 |
Target: 5'- gUCGCGCACaCCGUCuu-GGUgCCCAgCg -3' miRNA: 3'- -AGCGCGUGcGGCAGucuCUA-GGGUgG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 21506 | 0.72 | 0.521376 |
Target: 5'- uUCGuCGgGCGCCG-CAGcGuuUCCCACCa -3' miRNA: 3'- -AGC-GCgUGCGGCaGUCuCu-AGGGUGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 36982 | 0.78 | 0.228938 |
Target: 5'- gUUGCGcCGCGuUCGaUAGAGAUCCCACCa -3' miRNA: 3'- -AGCGC-GUGC-GGCaGUCUCUAGGGUGG- -5' |
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30581 | 3' | -57.6 | NC_006549.1 | + | 9819 | 1.13 | 0.001173 |
Target: 5'- aUCGCGCACGCCGUCAGAGAUCCCACCg -3' miRNA: 3'- -AGCGCGUGCGGCAGUCUCUAGGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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