Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30583 | 3' | -56.1 | NC_006549.1 | + | 131683 | 0.66 | 0.924893 |
Target: 5'- gCGGGCgGAGUGAaaGCgCGcuCACCGc -3' miRNA: 3'- -GCCCGgUUCACUgcUG-GCuuGUGGCc -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 21245 | 0.66 | 0.919442 |
Target: 5'- uCGGGCgGcauGGgcagGGCGGCCGAGguguuguCCGGa -3' miRNA: 3'- -GCCCGgU---UCa---CUGCUGGCUUgu-----GGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 50509 | 0.66 | 0.919442 |
Target: 5'- uGGGuCCAGGUGGaccaGCCGGGCcauCUGGc -3' miRNA: 3'- gCCC-GGUUCACUgc--UGGCUUGu--GGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 6628 | 0.66 | 0.91376 |
Target: 5'- cCGGGCCGAcugGAauACCGGGuccCACUGGg -3' miRNA: 3'- -GCCCGGUUca-CUgcUGGCUU---GUGGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 7722 | 0.66 | 0.91376 |
Target: 5'- gGGGCCcAG-GGgGGCCGAugGgCCGc -3' miRNA: 3'- gCCCGGuUCaCUgCUGGCUugU-GGCc -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 98159 | 0.66 | 0.91376 |
Target: 5'- uGGGCaGAGUGACGcACCc--CGCCaGGa -3' miRNA: 3'- gCCCGgUUCACUGC-UGGcuuGUGG-CC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 74438 | 0.67 | 0.888746 |
Target: 5'- aCGGGCaacaGAGaGGCGGCCguaaGAGCAgUGGu -3' miRNA: 3'- -GCCCGg---UUCaCUGCUGG----CUUGUgGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 101967 | 0.67 | 0.867669 |
Target: 5'- uGGGCCAGGaGACc-CCGuuGCGCCaGGg -3' miRNA: 3'- gCCCGGUUCaCUGcuGGCu-UGUGG-CC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 102446 | 0.68 | 0.85258 |
Target: 5'- uGGGCCGGGggguccagGAgGGCCGGgguucACGCCu- -3' miRNA: 3'- gCCCGGUUCa-------CUgCUGGCU-----UGUGGcc -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 41634 | 0.68 | 0.828508 |
Target: 5'- gGGGuCCAGG-GACcACCGGGaccacagggacCACCGGg -3' miRNA: 3'- gCCC-GGUUCaCUGcUGGCUU-----------GUGGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 74840 | 0.68 | 0.828508 |
Target: 5'- aGGcCCGGGUGGCuaaucacuGCCGGACgGCCGGa -3' miRNA: 3'- gCCcGGUUCACUGc-------UGGCUUG-UGGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 41847 | 0.7 | 0.708963 |
Target: 5'- gCGGGCCcgcGAG-GACccCCGGggGCGCCGGg -3' miRNA: 3'- -GCCCGG---UUCaCUGcuGGCU--UGUGGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 7487 | 0.71 | 0.699078 |
Target: 5'- gGGGCCAagGGUGcCGAUgGAccUACCGGa -3' miRNA: 3'- gCCCGGU--UCACuGCUGgCUu-GUGGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 125748 | 0.71 | 0.699078 |
Target: 5'- uGcGGCCAGGgauccgaGACGACCGuacacgguucCGCCGGa -3' miRNA: 3'- gC-CCGGUUCa------CUGCUGGCuu--------GUGGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 51334 | 0.75 | 0.444836 |
Target: 5'- uGGGCCGuccacGUGugGACCGAaagaauuacuGC-CCGGg -3' miRNA: 3'- gCCCGGUu----CACugCUGGCU----------UGuGGCC- -5' |
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30583 | 3' | -56.1 | NC_006549.1 | + | 12858 | 1.09 | 0.003031 |
Target: 5'- uCGGGCCAAGUGACGACCGAACACCGGc -3' miRNA: 3'- -GCCCGGUUCACUGCUGGCUUGUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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