miRNA: 3'- ggUUGGUcu-GGUAUCCGUCgCACUag -5'
miRNA: 3'- -GGUUgGUc---UGGUaUCCGUCGCACUAG- -5'
miRNA: 3'- -GGUUGGUCUGGUAu-CCGu--CGCaCUag -5'
miRNA: 3'- gguUGGUCU-GGUAUCCGuCGCA--------------CUAG- -5'
miRNA: 3'- -GGUUGGUCUGGUAUCCGUCGCACUAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30586 | 5' | -52.4 | NC_006549.1 | + | 5450 | 0.66 | 0.979772 |
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Target: 5'- -uGACCAauuCUAUGGGCAGgGUGGc- -3' miRNA: 3'- ggUUGGUcu-GGUAUCCGUCgCACUag -5' |
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| 30586 | 5' | -52.4 | NC_006549.1 | + | 87896 | 0.68 | 0.931721 |
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Target: 5'- cUCAGgCAcaaaACCAcAGGCGGCGUGGUUu -3' miRNA: 3'- -GGUUgGUc---UGGUaUCCGUCGCACUAG- -5' |
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| 30586 | 5' | -52.4 | NC_006549.1 | + | 50496 | 0.68 | 0.920671 |
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Target: 5'- aCCAGCCGGGCCAUcuGGCcccGCG-GGg- -3' miRNA: 3'- -GGUUGGUCUGGUAu-CCGu--CGCaCUag -5' |
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| 30586 | 5' | -52.4 | NC_006549.1 | + | 92061 | 0.68 | 0.918926 |
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Target: 5'- --cACCAGAgCCAUGGGC-GCGUuggaacugaacggaGAUCa -3' miRNA: 3'- gguUGGUCU-GGUAUCCGuCGCA--------------CUAG- -5' |
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| 30586 | 5' | -52.4 | NC_006549.1 | + | 33122 | 1.12 | 0.0033 |
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Target: 5'- uCCAACCAGACCAUAGGCAGCGUGAUCg -3' miRNA: 3'- -GGUUGGUCUGGUAUCCGUCGCACUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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