Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30588 | 3' | -51.7 | NC_006549.1 | + | 110349 | 0.66 | 0.985766 |
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Target: 5'- cGUCaGAAuacGCUUUUGG--GCGCcCGCCa -3' miRNA: 3'- uCAGcCUU---CGAAGACUuaUGCG-GCGG- -5' |
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| 30588 | 3' | -51.7 | NC_006549.1 | + | 130613 | 0.66 | 0.985766 |
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Target: 5'- uGUCGGAAcg-UCUGGAaggagugGCGCUGCa -3' miRNA: 3'- uCAGCCUUcgaAGACUUa------UGCGGCGg -5' |
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| 30588 | 3' | -51.7 | NC_006549.1 | + | 134762 | 0.66 | 0.983943 |
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Target: 5'- cAGUCGGuaAAGCcgagCgGggUGCGCCauccguaguuaGCCa -3' miRNA: 3'- -UCAGCC--UUCGaa--GaCuuAUGCGG-----------CGG- -5' |
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| 30588 | 3' | -51.7 | NC_006549.1 | + | 63316 | 0.68 | 0.963173 |
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Target: 5'- uGUUGGAAGUUcucuugcacaacCUGGAUACGCCaacauuaggaucgacGCCg -3' miRNA: 3'- uCAGCCUUCGAa-----------GACUUAUGCGG---------------CGG- -5' |
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| 30588 | 3' | -51.7 | NC_006549.1 | + | 55642 | 0.7 | 0.914763 |
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Target: 5'- gAGUCuGGAGGCUcaacaUGcccguuUugGCCGCCa -3' miRNA: 3'- -UCAG-CCUUCGAag---ACuu----AugCGGCGG- -5' |
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| 30588 | 3' | -51.7 | NC_006549.1 | + | 36563 | 1.13 | 0.004035 |
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Target: 5'- gAGUCGGAAGCUUCUGAAUACGCCGCCu -3' miRNA: 3'- -UCAGCCUUCGAAGACUUAUGCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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