Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30589 | 5' | -52.3 | NC_006549.1 | + | 119628 | 0.66 | 0.973603 |
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Target: 5'- gGGAGCUGGGGGCG-GAggGgcgUGCUGu -3' miRNA: 3'- aCUUUGGCCUCCGCaCUaaCa--GUGGC- -5' |
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| 30589 | 5' | -52.3 | NC_006549.1 | + | 21981 | 0.68 | 0.934909 |
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Target: 5'- uUGcgGCCGGAGGCGguugUGUuuugCGCCa -3' miRNA: 3'- -ACuuUGGCCUCCGCacuaACA----GUGGc -5' |
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| 30589 | 5' | -52.3 | NC_006549.1 | + | 90945 | 0.69 | 0.912593 |
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Target: 5'- uUGGAACgGGuGGUGgggcGAUUGUCGuuGg -3' miRNA: 3'- -ACUUUGgCCuCCGCa---CUAACAGUggC- -5' |
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| 30589 | 5' | -52.3 | NC_006549.1 | + | 83501 | 0.69 | 0.906388 |
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Target: 5'- cGGAACCGGAccugacGGcCGUGGUgcccGUgGCCGu -3' miRNA: 3'- aCUUUGGCCU------CC-GCACUAa---CAgUGGC- -5' |
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| 30589 | 5' | -52.3 | NC_006549.1 | + | 22752 | 0.7 | 0.879127 |
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Target: 5'- gGAGAUCGGAGGCGcGAUaacgGCCGa -3' miRNA: 3'- aCUUUGGCCUCCGCaCUAacagUGGC- -5' |
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| 30589 | 5' | -52.3 | NC_006549.1 | + | 37740 | 1.1 | 0.005253 |
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Target: 5'- cUGAAACCGGAGGCGUGAUUGUCACCGg -3' miRNA: 3'- -ACUUUGGCCUCCGCACUAACAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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