Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30592 | 3' | -45.6 | NC_006549.1 | + | 126982 | 0.66 | 0.999976 |
Target: 5'- --cGCAGAGGCGA--GCAugAC-CGCa -3' miRNA: 3'- ucaUGUCUUCGUUguUGUugUGaGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 138997 | 0.66 | 0.999976 |
Target: 5'- aGGuUGCAGAAGCAACcaaaAACAUUgAUg -3' miRNA: 3'- -UC-AUGUCUUCGUUGuug-UUGUGAgUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 35114 | 0.66 | 0.999966 |
Target: 5'- gGGUAUGGu-GCAcuACGACAucaaaGCUCACa -3' miRNA: 3'- -UCAUGUCuuCGU--UGUUGUug---UGAGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 96441 | 0.66 | 0.999938 |
Target: 5'- --aACAGAcGCAGCAAacucuaGACACuuuUCACa -3' miRNA: 3'- ucaUGUCUuCGUUGUUg-----UUGUG---AGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 78639 | 0.66 | 0.999938 |
Target: 5'- cGUACAGGauGGCcccGGCGACGGguCUCAg -3' miRNA: 3'- uCAUGUCU--UCG---UUGUUGUUguGAGUg -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 26732 | 0.67 | 0.99989 |
Target: 5'- cGUGCAcGGcuacAGUAaaGCAACAACAC-CGCa -3' miRNA: 3'- uCAUGU-CU----UCGU--UGUUGUUGUGaGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 115943 | 0.67 | 0.999737 |
Target: 5'- gGGcGCAG-AGCAcgGCAACAACGCagugccagacguuuUCACu -3' miRNA: 3'- -UCaUGUCuUCGU--UGUUGUUGUG--------------AGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 27603 | 0.68 | 0.999601 |
Target: 5'- uGUACGu-AGCu---GCAACACUCGCg -3' miRNA: 3'- uCAUGUcuUCGuuguUGUUGUGAGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 35758 | 0.68 | 0.99954 |
Target: 5'- aAGUACGGAcgcuucuucggacgaAGauuACGACGACAC-CGCg -3' miRNA: 3'- -UCAUGUCU---------------UCgu-UGUUGUUGUGaGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 139269 | 0.68 | 0.999366 |
Target: 5'- cGUGCGGAAcCGAaauuCAAUGCUCACa -3' miRNA: 3'- uCAUGUCUUcGUUguu-GUUGUGAGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 75658 | 0.69 | 0.997826 |
Target: 5'- uGGUGCAGAGaCAACAaccaaaaauGCAGCGC-CAUa -3' miRNA: 3'- -UCAUGUCUUcGUUGU---------UGUUGUGaGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 135601 | 0.71 | 0.993932 |
Target: 5'- --cGCAGuuaaaugucucGAGCAACAACAGCA-UCGCg -3' miRNA: 3'- ucaUGUC-----------UUCGUUGUUGUUGUgAGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 4420 | 0.71 | 0.992925 |
Target: 5'- ----aGGAGGCGuguuACAACcGCGCUCACa -3' miRNA: 3'- ucaugUCUUCGU----UGUUGuUGUGAGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 122550 | 0.71 | 0.992925 |
Target: 5'- --cGCAGcAGCGGCGACAGCcuuCUCAg -3' miRNA: 3'- ucaUGUCuUCGUUGUUGUUGu--GAGUg -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 126474 | 0.71 | 0.991789 |
Target: 5'- --gACGGuuGCAACGACAugacguGCGCUUACu -3' miRNA: 3'- ucaUGUCuuCGUUGUUGU------UGUGAGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 62245 | 0.72 | 0.987499 |
Target: 5'- gAGUACAGAAuGCug-GAgGACAUUCACg -3' miRNA: 3'- -UCAUGUCUU-CGuugUUgUUGUGAGUG- -5' |
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30592 | 3' | -45.6 | NC_006549.1 | + | 45567 | 1.12 | 0.015315 |
Target: 5'- cAGUACAGAAGCAACAACAACACUCACg -3' miRNA: 3'- -UCAUGUCUUCGUUGUUGUUGUGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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