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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30593 | 5' | -52.6 | NC_006549.1 | + | 10195 | 0.67 | 0.953628 |
Target: 5'- aGCUCAGa--CCCGCGCGGaagaaaUGCGa -3' miRNA: 3'- gCGAGUUgacGGGCGUGUCaaa---ACGC- -5' |
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30593 | 5' | -52.6 | NC_006549.1 | + | 113747 | 0.71 | 0.822101 |
Target: 5'- cCGUUUGcCUGCCCGCcGCGuUUUUGCGg -3' miRNA: 3'- -GCGAGUuGACGGGCG-UGUcAAAACGC- -5' |
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30593 | 5' | -52.6 | NC_006549.1 | + | 45989 | 1.09 | 0.004983 |
Target: 5'- aCGCUCAACUGCCCGCACAGUUUUGCGc -3' miRNA: 3'- -GCGAGUUGACGGGCGUGUCAAAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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