Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30594 | 3' | -60.3 | NC_006549.1 | + | 41010 | 0.66 | 0.726619 |
Target: 5'- aGGUCCgauagGCCCCCagg--GAUCCcuGGGGg -3' miRNA: 3'- -CCGGGa----CGGGGGguugaCUAGG--UCCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 51938 | 0.66 | 0.716922 |
Target: 5'- cGGCCCUGCCUUguaCGACaaGA-CCAGGc -3' miRNA: 3'- -CCGGGACGGGGg--GUUGa-CUaGGUCCu -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 41716 | 0.66 | 0.69437 |
Target: 5'- cGCCCgcggGCCCCCCGGCccccccggccccccUGggCCcgcgggcccagcAGGAg -3' miRNA: 3'- cCGGGa---CGGGGGGUUG--------------ACuaGG------------UCCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 50454 | 0.66 | 0.69437 |
Target: 5'- gGGaCCCacugGCCCCgCCGgaccggcggggcccAUUGGUCCGGGc -3' miRNA: 3'- -CC-GGGa---CGGGG-GGU--------------UGACUAGGUCCu -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 50226 | 0.67 | 0.654579 |
Target: 5'- gGGCCCUuggacgccaggagcGCCCauaucUCCGAUUGGUCCccuuGGAc -3' miRNA: 3'- -CCGGGA--------------CGGG-----GGGUUGACUAGGu---CCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 40889 | 0.67 | 0.637555 |
Target: 5'- aGCCUcgGCCCCCC---UGGUCCgaAGGGg -3' miRNA: 3'- cCGGGa-CGGGGGGuugACUAGG--UCCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 100232 | 0.67 | 0.637555 |
Target: 5'- aGGUCCcguUGCCCUCUGGCUGGggaccucgggcUUCGGGGg -3' miRNA: 3'- -CCGGG---ACGGGGGGUUGACU-----------AGGUCCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 113929 | 0.67 | 0.627533 |
Target: 5'- cGCCCUGCCCUCUAACaaauuugUgAGGAu -3' miRNA: 3'- cCGGGACGGGGGGUUGacua---GgUCCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 102370 | 0.68 | 0.587549 |
Target: 5'- gGGCCCgUGUCUCCCAugUcacccuuuGAUCCucgcGGAc -3' miRNA: 3'- -CCGGG-ACGGGGGGUugA--------CUAGGu---CCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 40593 | 0.7 | 0.472004 |
Target: 5'- aGGCCCccccggGCCCgCCGGCUua-CCAGGGa -3' miRNA: 3'- -CCGGGa-----CGGGgGGUUGAcuaGGUCCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 7386 | 0.73 | 0.295826 |
Target: 5'- gGGCCCcaggGCCCgCCCGGCcc-UCCAGGGc -3' miRNA: 3'- -CCGGGa---CGGG-GGGUUGacuAGGUCCU- -5' |
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30594 | 3' | -60.3 | NC_006549.1 | + | 50615 | 1.1 | 0.000851 |
Target: 5'- gGGCCCUGCCCCCCAACUGAUCCAGGAg -3' miRNA: 3'- -CCGGGACGGGGGGUUGACUAGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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