miRNA: 3'- gGUCGGUacaUGCCua---GGAACGUGGU- -5'
miRNA: 3'- gGUCGGUACaUGCCU---AGG---------------AACGUGGu -5'
miRNA: 3'- ggUCGGUACA--UGCCUAGGaacg--UGGu -5'
miRNA: 3'- gGUCGGUaCAU-GCCUAGGaa--C-GUGGu -5'
miRNA: 3'- -GGUCGGUACAUGCCUAGGAACGUGGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30607 | 5' | -53.2 | NC_006549.1 | + | 21979 | 0.67 | 0.949015 |
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Target: 5'- gCGGCCGgagGCGGuugugUUUUGCGCCAg -3' miRNA: 3'- gGUCGGUacaUGCCua---GGAACGUGGU- -5' |
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| 30607 | 5' | -53.2 | NC_006549.1 | + | 52716 | 0.67 | 0.936919 |
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Target: 5'- gCGGCCAUG-GCGGGcgaUCCgucagcguuggcggcUUGCGCCc -3' miRNA: 3'- gGUCGGUACaUGCCU---AGG---------------AACGUGGu -5' |
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| 30607 | 5' | -53.2 | NC_006549.1 | + | 74530 | 0.68 | 0.918548 |
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Target: 5'- -gGGCCGUGUggGCGGAUCCgucaaaACCc -3' miRNA: 3'- ggUCGGUACA--UGCCUAGGaacg--UGGu -5' |
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| 30607 | 5' | -53.2 | NC_006549.1 | + | 68268 | 0.68 | 0.912593 |
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Target: 5'- aCAGCCGcGUGuCGGGUCCggugGaCGCCc -3' miRNA: 3'- gGUCGGUaCAU-GCCUAGGaa--C-GUGGu -5' |
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| 30607 | 5' | -53.2 | NC_006549.1 | + | 78884 | 1.11 | 0.003426 |
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Target: 5'- cCCAGCCAUGUACGGAUCCUUGCACCAg -3' miRNA: 3'- -GGUCGGUACAUGCCUAGGAACGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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