Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30608 | 5' | -59.6 | NC_006549.1 | + | 86153 | 0.66 | 0.725984 |
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Target: 5'- cAAUCgCCCGAcgAGgGGCaguucuGGCCGGACGu- -3' miRNA: 3'- -UUAG-GGGCU--UCgCCG------CCGGUCUGUcg -5' |
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| 30608 | 5' | -59.6 | NC_006549.1 | + | 53299 | 0.67 | 0.676282 |
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Target: 5'- ---aCCCGAAgaggccGCGGCGGgagaagacCCAGcCAGCa -3' miRNA: 3'- uuagGGGCUU------CGCCGCC--------GGUCuGUCG- -5' |
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| 30608 | 5' | -59.6 | NC_006549.1 | + | 7731 | 0.67 | 0.645964 |
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Target: 5'- aGGUCCCCaGggGCccagGGgGGCCgauGGGCcGCa -3' miRNA: 3'- -UUAGGGG-CuuCG----CCgCCGG---UCUGuCG- -5' |
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| 30608 | 5' | -59.6 | NC_006549.1 | + | 66287 | 0.67 | 0.625684 |
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Target: 5'- -cUCUUgGucGCGacGCGGCCAGACAGa -3' miRNA: 3'- uuAGGGgCuuCGC--CGCCGGUCUGUCg -5' |
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| 30608 | 5' | -59.6 | NC_006549.1 | + | 52750 | 0.68 | 0.614535 |
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Target: 5'- -uUCCCCGucgcaggaggaacAAGCGGCGGagCGcGCGGCc -3' miRNA: 3'- uuAGGGGC-------------UUCGCCGCCg-GUcUGUCG- -5' |
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| 30608 | 5' | -59.6 | NC_006549.1 | + | 34031 | 0.68 | 0.59532 |
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Target: 5'- cGUCCCCGAcGaCGGCGggugguucGCCAcGAgGGCu -3' miRNA: 3'- uUAGGGGCUuC-GCCGC--------CGGU-CUgUCG- -5' |
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| 30608 | 5' | -59.6 | NC_006549.1 | + | 96145 | 0.69 | 0.535476 |
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Target: 5'- --aCCCUGGAGCGGUagcGGCC--GCGGUg -3' miRNA: 3'- uuaGGGGCUUCGCCG---CCGGucUGUCG- -5' |
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| 30608 | 5' | -59.6 | NC_006549.1 | + | 128087 | 0.69 | 0.52569 |
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Target: 5'- ---aCCC-AAGUGGUGGCUAGACuuuGCa -3' miRNA: 3'- uuagGGGcUUCGCCGCCGGUCUGu--CG- -5' |
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| 30608 | 5' | -59.6 | NC_006549.1 | + | 79505 | 1.08 | 0.00121 |
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Target: 5'- uAAUCCCCGAAGCGGCGGCCAGACAGCu -3' miRNA: 3'- -UUAGGGGCUUCGCCGCCGGUCUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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