Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30610 | 3' | -62.3 | NC_006549.1 | + | 21236 | 0.66 | 0.663313 |
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Target: 5'- aUGGGCagGGCGGCCGaGGUGUuguCCGg -3' miRNA: 3'- gGCCUGgaCUGCCGGCaCCACG---GGCa -5' |
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| 30610 | 3' | -62.3 | NC_006549.1 | + | 7049 | 0.66 | 0.643856 |
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Target: 5'- gCCGGACCccGCGGaccCCGcGG-GCCCGc -3' miRNA: 3'- -GGCCUGGacUGCC---GGCaCCaCGGGCa -5' |
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| 30610 | 3' | -62.3 | NC_006549.1 | + | 49677 | 0.66 | 0.643856 |
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Target: 5'- -gGGACCUuGCGGaCCcacGGUGCCCu- -3' miRNA: 3'- ggCCUGGAcUGCC-GGca-CCACGGGca -5' |
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| 30610 | 3' | -62.3 | NC_006549.1 | + | 7940 | 0.66 | 0.614625 |
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Target: 5'- cCCGGACCcGAUGgggaGCCG-GG-GCCUGa -3' miRNA: 3'- -GGCCUGGaCUGC----CGGCaCCaCGGGCa -5' |
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| 30610 | 3' | -62.3 | NC_006549.1 | + | 7463 | 0.66 | 0.614625 |
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Target: 5'- aCCGGACCaGAUGGCgaccCG-GGUGCUaaCGg -3' miRNA: 3'- -GGCCUGGaCUGCCG----GCaCCACGG--GCa -5' |
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| 30610 | 3' | -62.3 | NC_006549.1 | + | 50801 | 0.67 | 0.604897 |
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Target: 5'- uUGGGuCCUGGCGGgcCCGcUGG-GCCUGUu -3' miRNA: 3'- gGCCU-GGACUGCC--GGC-ACCaCGGGCA- -5' |
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| 30610 | 3' | -62.3 | NC_006549.1 | + | 49751 | 0.69 | 0.494854 |
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Target: 5'- gCGGagccuGCCUGACccugcgucccccgcgGGcCCGUGGgGCCCGUu -3' miRNA: 3'- gGCC-----UGGACUG---------------CC-GGCACCaCGGGCA- -5' |
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| 30610 | 3' | -62.3 | NC_006549.1 | + | 64014 | 0.7 | 0.396425 |
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Target: 5'- -aGGGCCgUGGCGuaCGcGGUGCCCGc -3' miRNA: 3'- ggCCUGG-ACUGCcgGCaCCACGGGCa -5' |
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| 30610 | 3' | -62.3 | NC_006549.1 | + | 83497 | 1.09 | 0.000851 |
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Target: 5'- aCCGGACCUGACGGCCGUGGUGCCCGUg -3' miRNA: 3'- -GGCCUGGACUGCCGGCACCACGGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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