Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30610 | 5' | -55.3 | NC_006549.1 | + | 40941 | 0.66 | 0.895509 |
Target: 5'- -cACGGGCCccccggGCCCgauGGGGCCGa-- -3' miRNA: 3'- uuUGCCCGGa-----UGGGau-UUUCGGCggu -5' |
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30610 | 5' | -55.3 | NC_006549.1 | + | 50248 | 0.67 | 0.853992 |
Target: 5'- cAGGCGGGCCagcgucucccacggGCCCUu--GGaCGCCAg -3' miRNA: 3'- -UUUGCCCGGa-------------UGGGAuuuUCgGCGGU- -5' |
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30610 | 5' | -55.3 | NC_006549.1 | + | 50991 | 0.67 | 0.842527 |
Target: 5'- ---aGGGCCgaucgGCCCUAAGGGgC-CCAu -3' miRNA: 3'- uuugCCCGGa----UGGGAUUUUCgGcGGU- -5' |
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30610 | 5' | -55.3 | NC_006549.1 | + | 41849 | 0.69 | 0.740537 |
Target: 5'- uAGCGGGCCcgcgaggACCCccGGGGgCGCCGg -3' miRNA: 3'- uUUGCCCGGa------UGGGauUUUCgGCGGU- -5' |
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30610 | 5' | -55.3 | NC_006549.1 | + | 3581 | 0.71 | 0.6475 |
Target: 5'- -cGCGGGCCU-CgCUAAGGGUauaGCCAu -3' miRNA: 3'- uuUGCCCGGAuGgGAUUUUCGg--CGGU- -5' |
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30610 | 5' | -55.3 | NC_006549.1 | + | 53451 | 0.71 | 0.626441 |
Target: 5'- cGAGCGGGCg-GCCgaAgagauugcgGAAGCCGCCAu -3' miRNA: 3'- -UUUGCCCGgaUGGgaU---------UUUCGGCGGU- -5' |
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30610 | 5' | -55.3 | NC_006549.1 | + | 83462 | 0.95 | 0.022886 |
Target: 5'- aAAACGGGCUcACCCUAAAAGCCGCCAu -3' miRNA: 3'- -UUUGCCCGGaUGGGAUUUUCGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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