Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30611 | 5' | -60.1 | NC_006549.1 | + | 33149 | 0.66 | 0.717013 |
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Target: 5'- gCGCACacUGCGGUGUGGGUGUA-CGAa -3' miRNA: 3'- -GCGUGc-GCGUUACGCCCGCGUgGCUa -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 5441 | 0.66 | 0.687314 |
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Target: 5'- -cUAUGgGCAGggugGCGGGCGCGCUa-- -3' miRNA: 3'- gcGUGCgCGUUa---CGCCCGCGUGGcua -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 97754 | 0.67 | 0.657213 |
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Target: 5'- uGCAaGaCGCGGUGCuGGGCGCGCa--- -3' miRNA: 3'- gCGUgC-GCGUUACG-CCCGCGUGgcua -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 91380 | 0.67 | 0.626936 |
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Target: 5'- cCGCACGggaCGCAGacgGUGGGCGCagACCa-- -3' miRNA: 3'- -GCGUGC---GCGUUa--CGCCCGCG--UGGcua -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 127693 | 0.67 | 0.626936 |
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Target: 5'- aCGUuCGCGCAAcUGUcuGuGCGCGCCGAUg -3' miRNA: 3'- -GCGuGCGCGUU-ACGc-C-CGCGUGGCUA- -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 120204 | 0.67 | 0.616841 |
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Target: 5'- aCGCACGCGgAucguUGCGGGUcacgaagggcugGCGCCc-- -3' miRNA: 3'- -GCGUGCGCgUu---ACGCCCG------------CGUGGcua -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 30800 | 0.68 | 0.586652 |
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Target: 5'- uGCGCGCGCGA-GUGGGUcaaccCGCCGu- -3' miRNA: 3'- gCGUGCGCGUUaCGCCCGc----GUGGCua -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 60468 | 0.71 | 0.425352 |
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Target: 5'- gGCGCcCGCGAcggcguugGCGGuGUGCGCCGAUa -3' miRNA: 3'- gCGUGcGCGUUa-------CGCC-CGCGUGGCUA- -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 53469 | 0.72 | 0.351257 |
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Target: 5'- gCGCauACGCGCGAgagacgaGCGGGCG-GCCGAa -3' miRNA: 3'- -GCG--UGCGCGUUa------CGCCCGCgUGGCUa -5' |
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| 30611 | 5' | -60.1 | NC_006549.1 | + | 91530 | 1.05 | 0.001991 |
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Target: 5'- uCGCACGCGCAAUGCGGGCGCACCGAUc -3' miRNA: 3'- -GCGUGCGCGUUACGCCCGCGUGGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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