Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30613 | 3' | -55.3 | NC_006549.1 | + | 15052 | 0.66 | 0.899827 |
Target: 5'- --gGUaucCUAGGGUCUCCuGUUUCugugGCCg -3' miRNA: 3'- caaCGc--GGUCCCAGAGGuCAAAG----CGG- -5' |
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30613 | 3' | -55.3 | NC_006549.1 | + | 102447 | 0.69 | 0.787928 |
Target: 5'- -cUGgGCCGGGGggUCCAGgaggGCCg -3' miRNA: 3'- caACgCGGUCCCagAGGUCaaagCGG- -5' |
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30613 | 3' | -55.3 | NC_006549.1 | + | 101996 | 0.72 | 0.606972 |
Target: 5'- -cUGgGCCcuGGUUgaCCAGUUUCGCCc -3' miRNA: 3'- caACgCGGucCCAGa-GGUCAAAGCGG- -5' |
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30613 | 3' | -55.3 | NC_006549.1 | + | 101952 | 1.11 | 0.002121 |
Target: 5'- cGUUGCGCCAGGGUCUCCAGUUUCGCCc -3' miRNA: 3'- -CAACGCGGUCCCAGAGGUCAAAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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