Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30614 | 3' | -56.6 | NC_006549.1 | + | 71612 | 0.66 | 0.86755 |
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Target: 5'- uACGAGUGGAAGCaGGUUcaauuccUugUCGCUGUa -3' miRNA: 3'- -UGCUCGCCUUUG-CCGA-------AugGGCGGUA- -5' |
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| 30614 | 3' | -56.6 | NC_006549.1 | + | 56352 | 0.66 | 0.860687 |
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Target: 5'- cGCGAcCGGAcGCGGCUUuccaACCgGCUg- -3' miRNA: 3'- -UGCUcGCCUuUGCCGAA----UGGgCGGua -5' |
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| 30614 | 3' | -56.6 | NC_006549.1 | + | 131684 | 0.67 | 0.78354 |
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Target: 5'- gGCGGGCGGAgugaaAGCGcGCUca-CCGCCc- -3' miRNA: 3'- -UGCUCGCCU-----UUGC-CGAaugGGCGGua -5' |
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| 30614 | 3' | -56.6 | NC_006549.1 | + | 53453 | 0.69 | 0.685022 |
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Target: 5'- gACGAGCGG--GCGGCcgaagagaUUGCggaagCCGCCAUg -3' miRNA: 3'- -UGCUCGCCuuUGCCG--------AAUG-----GGCGGUA- -5' |
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| 30614 | 3' | -56.6 | NC_006549.1 | + | 82807 | 0.69 | 0.685022 |
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Target: 5'- -gGAGCGGGcuuGACGGCg--UCCGCCu- -3' miRNA: 3'- ugCUCGCCU---UUGCCGaauGGGCGGua -5' |
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| 30614 | 3' | -56.6 | NC_006549.1 | + | 7139 | 0.7 | 0.62315 |
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Target: 5'- -gGAGCGGAuACGGuCUUGuuugguUCCGCCGg -3' miRNA: 3'- ugCUCGCCUuUGCC-GAAU------GGGCGGUa -5' |
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| 30614 | 3' | -56.6 | NC_006549.1 | + | 30800 | 0.72 | 0.541284 |
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Target: 5'- uGCGcGCGcGAGugGGUcaACCCGCCGUc -3' miRNA: 3'- -UGCuCGC-CUUugCCGaaUGGGCGGUA- -5' |
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| 30614 | 3' | -56.6 | NC_006549.1 | + | 5143 | 0.72 | 0.521313 |
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Target: 5'- cCGGGCGuGGGAgGGC-UGCUCGCCAUu -3' miRNA: 3'- uGCUCGC-CUUUgCCGaAUGGGCGGUA- -5' |
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| 30614 | 3' | -56.6 | NC_006549.1 | + | 105302 | 1.06 | 0.003326 |
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Target: 5'- gACGAGCGGAAACGGCUUACCCGCCAUg -3' miRNA: 3'- -UGCUCGCCUUUGCCGAAUGGGCGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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