Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30614 | 5' | -46.2 | NC_006549.1 | + | 24503 | 0.66 | 0.99994 |
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Target: 5'- cACGCGACGUGCuaugUCGGAaauUCUUg---- -3' miRNA: 3'- -UGUGCUGCACGu---GGCUU---AGAAauaac -5' |
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| 30614 | 5' | -46.2 | NC_006549.1 | + | 70416 | 0.67 | 0.999696 |
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Target: 5'- uACAUGGCGgGCACgGAgccagucAUCUggGUUGg -3' miRNA: 3'- -UGUGCUGCaCGUGgCU-------UAGAaaUAAC- -5' |
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| 30614 | 5' | -46.2 | NC_006549.1 | + | 65962 | 0.68 | 0.999524 |
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Target: 5'- uACACGACGguUGCugCGcGUCUUc---- -3' miRNA: 3'- -UGUGCUGC--ACGugGCuUAGAAauaac -5' |
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| 30614 | 5' | -46.2 | NC_006549.1 | + | 24018 | 0.7 | 0.997093 |
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Target: 5'- aGCuuGACGUGCACCGcAAgcUCUgguUUGg -3' miRNA: 3'- -UGugCUGCACGUGGC-UU--AGAaauAAC- -5' |
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| 30614 | 5' | -46.2 | NC_006549.1 | + | 91907 | 0.73 | 0.973096 |
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Target: 5'- -gACGACGUGCccAUUGAAUCUUUAg-- -3' miRNA: 3'- ugUGCUGCACG--UGGCUUAGAAAUaac -5' |
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| 30614 | 5' | -46.2 | NC_006549.1 | + | 105268 | 1.09 | 0.026143 |
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Target: 5'- aACACGACGUGCACCGAAUCUUUAUUGc -3' miRNA: 3'- -UGUGCUGCACGUGGCUUAGAAAUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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