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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30619 | 5' | -54.1 | NC_006549.1 | + | 56902 | 0.66 | 0.939252 |
Target: 5'- aAACCGgcgGC-CGCUgc-GGGCAGGGUGu -3' miRNA: 3'- -UUGGCa--UGaGCGAagaCUCGUCUCGC- -5' |
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30619 | 5' | -54.1 | NC_006549.1 | + | 11397 | 0.7 | 0.755109 |
Target: 5'- gAACCGca-UCGCUUCUcccAGCAGAGCc -3' miRNA: 3'- -UUGGCaugAGCGAAGAc--UCGUCUCGc -5' |
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30619 | 5' | -54.1 | NC_006549.1 | + | 116972 | 1.08 | 0.004149 |
Target: 5'- gAACCGUACUCGCUUCUGAGCAGAGCGg -3' miRNA: 3'- -UUGGCAUGAGCGAAGACUCGUCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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