Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30622 | 5' | -59.2 | NC_006549.1 | + | 2755 | 0.66 | 0.789148 |
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Target: 5'- uGCGCaacgGCUCGCCGCguuucgucaugacgCCA-GCGUACu- -3' miRNA: 3'- uCGCG----CGAGUGGCG--------------GGUaCGCAUGcc -5' |
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| 30622 | 5' | -59.2 | NC_006549.1 | + | 98968 | 0.67 | 0.707156 |
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Target: 5'- -cCGCGCUgGCCGCguuuuccaCGUGCGaaaacUGCGGa -3' miRNA: 3'- ucGCGCGAgUGGCGg-------GUACGC-----AUGCC- -5' |
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| 30622 | 5' | -59.2 | NC_006549.1 | + | 7770 | 0.69 | 0.567717 |
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Target: 5'- cAGCGCGUccUCgggGCCGCCag-GCGUACa- -3' miRNA: 3'- -UCGCGCG--AG---UGGCGGguaCGCAUGcc -5' |
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| 30622 | 5' | -59.2 | NC_006549.1 | + | 3184 | 0.71 | 0.453815 |
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Target: 5'- uGCGCGC-CACCGCCagcGUGUACu- -3' miRNA: 3'- uCGCGCGaGUGGCGGguaCGCAUGcc -5' |
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| 30622 | 5' | -59.2 | NC_006549.1 | + | 76376 | 0.74 | 0.345851 |
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Target: 5'- uGGCGCGCaaucgCACaCGUCCGcacacacagGCGUACGGa -3' miRNA: 3'- -UCGCGCGa----GUG-GCGGGUa--------CGCAUGCC- -5' |
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| 30622 | 5' | -59.2 | NC_006549.1 | + | 92166 | 0.77 | 0.223473 |
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Target: 5'- cGCGcCGCUUACCGUcgauuccgacgCCAUaGCGUGCGGg -3' miRNA: 3'- uCGC-GCGAGUGGCG-----------GGUA-CGCAUGCC- -5' |
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| 30622 | 5' | -59.2 | NC_006549.1 | + | 131670 | 1.1 | 0.00118 |
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Target: 5'- aAGCGCGCUCACCGCCCAUGCGUACGGa -3' miRNA: 3'- -UCGCGCGAGUGGCGGGUACGCAUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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